Overview

  • Product name

    Goat Anti-Mouse IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP), pre-adsorbed
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Minimal
    cross-reactivity


    Human, Rat more details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).
ICC Use at an assay dependent dilution.

References

ab98717 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody. If there is anything else I can do, please let me kn

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Answer

Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see clearly the regions that the other antibodies you selected bind too.   The difference between the preabsored and non preabsorbed i antibodies s that preabsorbed antibodies reduce non-specific background staining, especially in IHC when working with closely homologous species. If there is anything else I can help you with, please let me know.  

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Answer

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab98717 as a free of charge replacement. I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

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