Goat Anti-Mouse IgG heavy chain (10nm Gold) preadsorbed (ab27230)

Overview

  • Product name
    Goat Anti-Mouse IgG heavy chain (10nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Full length Mouse IgG.

  • Conjugation
    Gold 10nm

Properties

Applications

Our Abpromise guarantee covers the use of ab27230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab27230 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply and I am sorry for the misunderstanding.

    I can confirm that on average there are 12 antibody IgG molecules attached to each gold nanoparticle.

    The molecular weight of each antibody IgG molecule is approximately 150 kDa (˜2.99 x10-19g). The mass of one gold nanoparticle of diameter 10nm is approximately 1.01 x 10-17g.

    Adding together the mass of the gold nanoparticle and 12 antibody molecules we get a conjugate mass of 1.31 x 10-17g. This is equivalent to 7882 kDa for the whole complex and ca 6082 kDa for the gold particle.

    I hope this information is helpful.

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    Answer

    Thank you for your inquiry.

    Antibody (or immunoglobulin) molecules are glycoproteins composed of one or more units, each containing four polypeptide chains: two identical heavy chains (H) and two identical light chains (L).

    Each heavy chain has about twice the number of amino acids and molecular weight (˜50,000, 50kDa) as each light chain (˜25,000, 25kDa), resulting in a total immunoglobulin monomer molecular weight of approximately 150,000 (150kDa).

    I hope this answers your customers question and wish you a good weekend.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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