Goat Anti-Mouse IgG H&L (10nm Gold) (ab39619)

Submit a review Q&A (1)References (6)

Key features and details

  • Goat Anti-Mouse IgG H&L (10nm Gold)
  • Conjugation: Gold 10nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Electron Microscopy

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (10nm Gold)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: Electron Microscopymore details

  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 10 %.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Notes
Application notes
Electron Microscopy: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References (6)

Publishing research using ab39619? Please let us know so that we can cite the reference in this datasheet.

ab39619 has been referenced in 6 publications.

  • Shinde A  et al. Transglutaminase-2 facilitates extracellular vesicle-mediated establishment of the metastatic niche. Oncogenesis 9:16 (2020). PubMed: 32054828
  • Blanco-Rodriguez G  et al. Remodeling of the Core Leads HIV-1 Preintegration Complex into the Nucleus of Human Lymphocytes. J Virol 94:N/A (2020). PubMed: 32238582
  • Kong B  et al. Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome. Nat Commun 10:185 (2019). PubMed: 30643128
  • Varcianna A  et al. Micro-RNAs secreted through astrocyte-derived extracellular vesicles cause neuronal network degeneration in C9orf72 ALS. EBioMedicine 40:626-635 (2019). PubMed: 30711519
  • Watson DC  et al. Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes. J Extracell Vesicles 7:1442088 (2018). PubMed: 29535850
  • Nash LA  et al. Survival Motor Neuron Protein is Released from Cells in Exosomes: A Potential Biomarker for Spinal Muscular Atrophy. Sci Rep 7:13859 (2017). Electron Microscopy . PubMed: 29066780

Customer reviews and Q&As

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Question

May I ask a quotation of Goat polyclonal Secondary Antibody to Mouse IgG - H&L (6nm Gold) (ab39614)? My primer antibody is mouse anti human Integrin b1. Is it possible for me to use this as the secondary antibody to detect my integrin under FESEM? The FESEM resolution is 20 nm, do I need to purchase any enhancing reagent such as gold enhancement? Or you will recommend I use 20 nm gold? I am afraid if the gold is too large, I will cover my integrin. Thanks a lot.

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Answer

I was informed that the 6nm gold particles will be visible in FESEM, however intensity of the signal (brightness of the white spots) will be low. A Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold) (ab39619) might be more appropriate. I have included a link with an article describing HIGH RESOLUTION BACKSCATTER ELECTRON (BSE) IMAGING OF IMMUNOGOLD WITH IN-LENS AND BELOW-THE-LENS FIELD EMISSION SCANNING ELECTRON MICROSCOPES www.ecmjournal.org/journal/smi/pdf/smi99-04.pdf I have done the proforma for ab39619.

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