Overview

  • Product name

    Goat Anti-Mouse IgG H&L (10nm Gold)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: Electron Microscopymore details
  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 10 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:

    • Kong B  et al. Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome. Nat Commun 10:185 (2019). Read more (PubMed: 30643128) »
    • Varcianna A  et al. Micro-RNAs secreted through astrocyte-derived extracellular vesicles cause neuronal network degeneration in C9orf72 ALS. EBioMedicine 40:626-635 (2019). Read more (PubMed: 30711519) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    I was informed that the 6nm gold particles will be visible in FESEM, however intensity of the signal (brightness of the white spots) will be low. A Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold) (ab39619) might be more appropriate. I have included a link with an article describing HIGH RESOLUTION BACKSCATTER ELECTRON (BSE) IMAGING OF IMMUNOGOLD WITH IN-LENS AND BELOW-THE-LENS FIELD EMISSION SCANNING ELECTRON MICROSCOPES www.ecmjournal.org/journal/smi/pdf/smi99-04.pdf I have done the proforma for ab39619.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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