Overview

  • Product name
    Goat Anti-Mouse IgG H&L (10nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Full length Mouse IgG (H&L).

  • Conjugation
    Gold 10nm

Properties

Applications

Our Abpromise guarantee covers the use of ab27241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). Electron Microscopy . Read more (PubMed: 27592818) »
    • Riopel M  et al. Ultrastructural and immunohistochemical analysis of the 8-20 week human fetal pancreas. Islets 6:e982949 (2014). IHC . Read more (PubMed: 25425025) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you very much for your call today and your interest in these antibodies.

    Since35654 has not been tested in EM, I can offer a discount off a future purchase if you buy ab35654 now, test it inEM, and submit feedback to us in the form of an Abreview. The discount would be worth1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab35654 in EM. I will then send a discount code. This code must be issued before purchasing ab35654 so please wait for my reply before ordering.

    2. Purchase ab35654 either by phone, fax, or online (www.abcam.com).

    3. Test it in EM.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab35654 turns out to be unsuitable for EM, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Re: Problems with Gold-conjugated secondary antibodies ab27241 and ab27242 in EM with neuroblastoma cells.

    Hi, thanks for getting back to me.

    The cells I use are neuroblastoma cells with rat origin. I transfect my cells with the GFP mutant synaptopHluorin, this enables me to image the hydrated cells by fluorescence microscopy.

    I then fix my cells with PFA and permeabilised with Triton (I have tried concentrations between 0.1- 1%)

    I then labelwith anti GFP primary antibody andgold conjugated secondary antibody to allow detection of the labelled areas in the hydrated cells by atmospheric SEM.

    Generally the results show single particles of gold scattered throughout the cell. Even in samples where I have attempted to label actin filaments of endogenous proteins I do not see localisation to specific cellular patterns. It has also been questioned that surely more gold particles should be observed, taking into account the abundance of cellular structures labelled by the primary antibody. (Fluorescence assay have been carried out to determine the specificity of the primary antibody and have shown perfect colocalisation.)

    I have seen better results when I permeabilse with 1% Triton, although here I used a new transfection agent, so far I have not been able to repeat these results.

    I think a problem may be in either the specificity of the gold conjugated secondary antibody or the penetration of the gold particles into the hydrate cells. To solve this I can see two options 1) use smaller gold particles. This would be ok in normal SEMs although the resolution of my current instrument for ASEM is ˜20nm, so I'm not too keen to use much smaller particles.

    2) Alternative methods in fixation/permeabilisation to allow easier passage.

    I would be very grateful for your thoughts on my problem.

    Thanks,

    Read More
    Answer

    Thank you very much for sending all these details which enable us to investigate the problem.

    Thank you also for your patience while I was inquiring with the laboratory.

    Firstly I can confirm that we have not had any otherreports of any problems with these towbatches ofantibodies mentioned.

    In regards to solve the problem, I would like firstly to ask you whether you have checked whether the primary antibody is working. Have you used this primary antibody already on similar fixed slides and obtained a good signal?

    In regards to two secondary antibodies, the laboratory recommends toreturnto using 1% Triton to rule out the change of transfection agent as being the cause of the issues.

    We can also recommend(on advice of the laboratory)to try using a smaller gold particle (as for exampleab27244) and silver enhance this to obtain a larger signal which should be visible under the ASEM that they are using. The product needed to carry out this silver enhancing step is commercially available. If you would like us to source it for you, please let me know and I see what I can do. The silver enhancing step consists of simply adding an equal volume of the supplied silver enhancer and initiator to the sample after incubation with the secondary antibody (the conjugate being used) has been carried out. The silver particles will then attach to the gold particles which are present in the sample, growing them to a larger size and therefore making them large enough to be visible under ASEM.

    In case that the suggestions will not improve the results, and the primary antibody is excluded as possible source of problem, we will be happy to replace or refund these antibodies for you in accordance with our Abpromise policy.

    Please do let me know if you have any questions or concerns.

    I am looking forward to hear back from you to know whether the problem has been resolved. Thank you for your cooperation.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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