Goat Anti-Mouse IgG H&L (5nm Gold) preadsorbed (ab27244)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (5nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Full length Mouse IgG (H&L)

  • Conjugation
    Gold 5nm

Properties

Applications

Our Abpromise guarantee covers the use of ab27244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab27244 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    When we receive the antibody we use to manufacture gold conjugate, the antibody concentration is stated by the manufacturer. We then use the volume of this antibody we use in the gold conjugate manufacture to determine the antibody concentration present in the conjugate. This is calculated for each batch to ensure the value given is accurate.

    The antibody supplied to us is typically affinity purified.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for contacting us.

    I have been in contact with the originator of these products. Unfortunately they do not have any direct experience doing these types of procedures with pre-conjugated products and cannot provide any further information.

    My colleagues and I believe that these procedures should be effective for removal of unwanted buffer additives from the antibody however we would likely recommend using the antibody concentration kit as it seems less likely that damage may occur. Our concentration kits may be found at:

    1 column:

    https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html

    3 columns:

    https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html

    Please remember that these products are covered by our Abpromise guarantee. We are happy to provide scientific support, replacement or refund should a product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=abpromise

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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