Goat Anti-Mouse IgG H&L (6nm Gold) (ab39614)

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Overview

  • Product name
    Goat Anti-Mouse IgG H&L (6nm Gold)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation
    Gold 6nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab39614 has not yet been referenced specifically in any publications.

    Publishing research using ab39614? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    May I ask a quotation of Goat polyclonal Secondary Antibody to Mouse IgG - H&L (6nm Gold) (ab39614)? My primer antibody is mouse anti human Integrin b1. Is it possible for me to use this as the secondary antibody to detect my integrin under FESEM? The FESEM resolution is 20 nm, do I need to purchase any enhancing reagent such as gold enhancement? Or you will recommend I use 20 nm gold? I am afraid if the gold is too large, I will cover my integrin. Thanks a lot.

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    Answer

    I was informed that the 6nm gold particles will be visible in FESEM, however intensity of the signal (brightness of the white spots) will be low. A Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold) (ab39619) might be more appropriate. I have included a link with an article describing HIGH RESOLUTION BACKSCATTER ELECTRON (BSE) IMAGING OF IMMUNOGOLD WITH IN-LENS AND BELOW-THE-LENS FIELD EMISSION SCANNING ELECTRON MICROSCOPES www.ecmjournal.org/journal/smi/pdf/smi99-04.pdf I have done the proforma for ab39619.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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