Goat Anti-Mouse IgG H&L (6nm Gold) (ab39614)

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Key features and details

  • Goat Anti-Mouse IgG H&L (6nm Gold)
  • Conjugation: Gold 6nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Electron Microscopy

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (6nm Gold)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: Electron Microscopymore details

  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation

    Gold 6nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Notes
Application notes
Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

Light Microscopy: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References (0)

Publishing research using ab39614? Please let us know so that we can cite the reference in this datasheet.

ab39614 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

May I ask a quotation of Goat polyclonal Secondary Antibody to Mouse IgG - H&L (6nm Gold) (ab39614)? My primer antibody is mouse anti human Integrin b1. Is it possible for me to use this as the secondary antibody to detect my integrin under FESEM? The FESEM resolution is 20 nm, do I need to purchase any enhancing reagent such as gold enhancement? Or you will recommend I use 20 nm gold? I am afraid if the gold is too large, I will cover my integrin. Thanks a lot.

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Answer

I was informed that the 6nm gold particles will be visible in FESEM, however intensity of the signal (brightness of the white spots) will be low. A Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold) (ab39619) might be more appropriate. I have included a link with an article describing HIGH RESOLUTION BACKSCATTER ELECTRON (BSE) IMAGING OF IMMUNOGOLD WITH IN-LENS AND BELOW-THE-LENS FIELD EMISSION SCANNING ELECTRON MICROSCOPES www.ecmjournal.org/journal/smi/pdf/smi99-04.pdf I have done the proforma for ab39619.

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