Goat Anti-Mouse IgG H&L (ab182017)
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- References
- Protocols
Overview
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Product nameGoat Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesMouse
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SpecificityThe antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Chicken IgY, less than 2%. Human IgG, less than 6%. Rabbit IgG, less than 7%. Rat IgG, less than 47%.
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Tested applicationsSuitable for: ELISA, WB, IHC-P, IPmore details
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Immunogen
The details of the immunogen for this antibody are not available.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityAffinity purified
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Purification notesImmunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
Applications
Our Abpromise guarantee covers the use of ab182017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/20000 - 1/200000. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Images
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ELISA - Goat Anti-Mouse IgG H&L (ab182017)Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.
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ELISA - Goat Anti-Mouse IgG H&L (ab182017)Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
Datasheets and documents
References
ab182017 has not yet been referenced specifically in any publications.
