Goat Anti-Mouse IgG H&L (ab182017)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, IHC-P, IP
Overview
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Product name
Goat Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Chicken IgY, less than 2%. Human IgG, less than 6%. Rabbit IgG, less than 7%. Rat IgG, less than 47%. -
Tested applications
Suitable for: ELISA, WB, IHC-P, IPmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
Applications
Our Abpromise guarantee covers the use of ab182017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/20000 - 1/200000. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Images
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ELISA - Goat Anti-Mouse IgG H&L (ab182017)Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.
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ELISA - Goat Anti-Mouse IgG H&L (ab182017)Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab182017 has not yet been referenced specifically in any publications.
