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  1. Link

    goat-mouse-igg-hl-ab182017.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (ab182017)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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ELISA - Goat Anti-Mouse IgG H&L (ab182017)
  • ELISA - Goat Anti-Mouse IgG H&L (ab182017)

Key features and details

  • Goat Anti-Mouse IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, WB, IHC-P, IP

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Chicken IgY, less than 2%. Human IgG, less than 6%. Rabbit IgG, less than 7%. Rat IgG, less than 47%.
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IPmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Unconjugated

Associated products

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/200000.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
ELISA
1/20000 - 1/200000.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Images

  • ELISA - Goat Anti-Mouse IgG H&L (ab182017)
    ELISA - Goat Anti-Mouse IgG H&L (ab182017)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

  • ELISA - Goat Anti-Mouse IgG H&L (ab182017)
    ELISA - Goat Anti-Mouse IgG H&L (ab182017)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab182017? Please let us know so that we can cite the reference in this datasheet.

ab182017 has been referenced in 1 publication.

  • Zhang J  et al. TJP2 hepatobiliary disorders: Novel variants and clinical diversity. Hum Mutat 41:502-511 (2020). PubMed: 31696999

Customer reviews and Q&As

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