Overview

  • Product name
    Goat Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Mouse IgG, whole molecule

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/150000.
IP Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Conjugation Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/3000 - 1/15000.

Images

  • Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg

    Secondary
    Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution

    Observed band size: 28,55 kDa
    why is the actual band size different from the predicted?



    ab6708 incubation overnight at 4°C.

    Both heavy (55kDa) and light (28kDa) chains are detected.

  • All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

    Lane 1 : Mouse IgG (whole molecule)
    Lane 2 : Mouse IgG Fc fragment
    Lane 3 : Mouse IgG Fab fragment
    Lane 4 : Mouse IgM kappa
    Lane 5 : Mouse serum

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilution


    ab6708 incubated for 1 hour at room temperature.

References

This product has been referenced in:
See all 9 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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