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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (ab6708)

  • Datasheet
  • SDS
Submit a review Submit a question References (24)

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Western blot - Goat Anti-Mouse IgG H&L (ab6708)
  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)

Key features and details

  • Goat Anti-Mouse IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Mouse IgG, whole molecule

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Unconjugated

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6708 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/150000.
    IP
    Use at an assay dependent concentration.
    Immunodiffusion
    Use at an assay dependent concentration.
    Conjugation
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    WB
    1/3000 - 1/15000.
    Notes
    ELISA
    1/150000.
    IP
    Use at an assay dependent concentration.
    Immunodiffusion
    Use at an assay dependent concentration.
    Conjugation
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    WB
    1/3000 - 1/15000.

    Images

    • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
      Western blot - Goat Anti-Mouse IgG H&L (ab6708)
      Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg

      Secondary
      Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution

      Observed band size: 28,55 kDa
      why is the actual band size different from the predicted?



      ab6708 incubation overnight at 4°C.

      Both heavy (55kDa) and light (28kDa) chains are detected.

    • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
      Western blot - Goat Anti-Mouse IgG H&L (ab6708)
      All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

      Lane 1 : Mouse IgG (whole molecule)
      Lane 2 : Mouse IgG Fc fragment
      Lane 3 : Mouse IgG Fab fragment
      Lane 4 : Mouse IgM kappa
      Lane 5 : Mouse serum

      Lysates/proteins at 0.05 µg per lane.

      Secondary
      All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilution


      ab6708 incubated for 1 hour at room temperature.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (24)

    Publishing research using ab6708? Please let us know so that we can cite the reference in this datasheet.

    ab6708 has been referenced in 24 publications.

    • Feng Y  et al. BATF acts as an oncogene in non-small cell lung cancer. Oncol Lett 19:205-210 (2020). PubMed: 31897131
    • Li Y  et al. Erastin/sorafenib induces cisplatin-resistant non-small cell lung cancer cell ferroptosis through inhibition of the Nrf2/xCT pathway. Oncol Lett 19:323-333 (2020). PubMed: 31897145
    • Kuntze A  et al. Protonation of Piezo1 Impairs Cell-Matrix Interactions of Pancreatic Stellate Cells. Front Physiol 11:89 (2020). PubMed: 32116794
    • Li G  et al. Epididymal protein 3A is upregulated and promotes cell proliferation in non-small cell lung cancer. Oncol Lett 19:4024-4030 (2020). PubMed: 32391105
    • Tvrdonova M  et al. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Anal Bioanal Chem 411:559-564 (2019). PubMed: 30109381
    View all Publications for this product

    Customer reviews and Q&As

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