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  1. Link

    goat-mouse-igg-hl-ab6708.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (ab6708)

  • Datasheet
  • SDS
Submit a review Submit a question References (35)

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Western blot - Goat Anti-Mouse IgG H&L (ab6708)
  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)

Key features and details

  • Goat Anti-Mouse IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Mouse IgG, whole molecule

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Unconjugated

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/150000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
1/3000 - 1/15000.
Notes
ELISA
1/150000.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
1/3000 - 1/15000.

Images

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg

    Secondary
    Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution

    Observed band size: 28,55 kDa why is the actual band size different from the predicted?



    ab6708 incubation overnight at 4°C.

    Both heavy (55kDa) and light (28kDa) chains are detected.

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    Western blot - Goat Anti-Mouse IgG H&L (ab6708)
    All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

    Lane 1 : Mouse IgG (whole molecule)
    Lane 2 : Mouse IgG Fc fragment
    Lane 3 : Mouse IgG Fab fragment
    Lane 4 : Mouse IgM kappa
    Lane 5 : Mouse serum

    Lysates/proteins at 0.05 µg per lane.

    Secondary
    All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilution


    ab6708 incubated for 1 hour at room temperature.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (35)

Publishing research using ab6708? Please let us know so that we can cite the reference in this datasheet.

ab6708 has been referenced in 35 publications.

  • Kawanishi N & Machida S Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice. Muscle Nerve 63:600-607 (2021). PubMed: 33386611
  • Jeong MG  et al. Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay. Exp Mol Med 53:291-299 (2021). PubMed: 33603128
  • Xiao D  et al. Mechanisms of microRNA-142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. Int J Mol Med 47:N/A (2021). PubMed: 33846769
  • Kahriman A  et al. Mouse closed head traumatic brain injury replicates the histological tau pathology pattern of human disease: characterization of a novel model and systematic review of the literature. Acta Neuropathol Commun 9:118 (2021). PubMed: 34187585
  • Xie Q  et al. Proteome profiling of formalin-fixed, paraffin-embedded lung adenocarcinoma tissues using a tandem mass tag-based quantitative proteomics approach. Oncol Lett 22:706 (2021). PubMed: 34457061
View all Publications for this product

Customer reviews and Q&As

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