Goat Anti-Mouse IgG H&L (ab6708)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
Overview
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Product name
Goat Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details -
Immunogen
Mouse IgG, whole molecule
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/150000.
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| IP |
Use at an assay dependent concentration.
|
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| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| WB |
1/3000 - 1/15000.
|
| Notes |
|---|
|
ELISA
1/150000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/15000. |
Images
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (24)
ab6708 has been referenced in 24 publications.
- Feng Y et al. BATF acts as an oncogene in non-small cell lung cancer. Oncol Lett 19:205-210 (2020). PubMed: 31897131
- Li Y et al. Erastin/sorafenib induces cisplatin-resistant non-small cell lung cancer cell ferroptosis through inhibition of the Nrf2/xCT pathway. Oncol Lett 19:323-333 (2020). PubMed: 31897145
- Kuntze A et al. Protonation of Piezo1 Impairs Cell-Matrix Interactions of Pancreatic Stellate Cells. Front Physiol 11:89 (2020). PubMed: 32116794
- Li G et al. Epididymal protein 3A is upregulated and promotes cell proliferation in non-small cell lung cancer. Oncol Lett 19:4024-4030 (2020). PubMed: 32391105
- Tvrdonova M et al. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Anal Bioanal Chem 411:559-564 (2019). PubMed: 30109381
