Goat Anti-Mouse IgG H&L (ab6708)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameGoat Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesMouse
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Tested applicationsSuitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details
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Immunogen
Mouse IgG, whole molecule
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/150000. | |
| IP | Use at an assay dependent concentration. | |
| Immunodiffusion | Use at an assay dependent concentration. | |
| Conjugation | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/3000 - 1/15000. |
Images
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
References
This product has been referenced in:
- Zhou Z et al. Betulin induces cytochrome c release and apoptosis in colon cancer cells via NOXA. Oncol Lett 15:7319-7327 (2018). Read more (PubMed: 29725447) »
- Zhu Y et al. Knockout of SIRT4 decreases chemosensitivity to 5-FU in colorectal cancer cells. Oncol Lett 16:1675-1681 (2018). Read more (PubMed: 30008852) »
