• Product name
    Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488)
  • Host species
  • Target species
  • Specificity
    Specific to mouse IgG
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
  • Isotype
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab150113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

IHC-P Use at an assay dependent concentration.


  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150113 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • IHC - Wholemount of Caenorhabditis elegans embryo labelling RNA polymerase II CTD repeat YSPTSPS with ab817. Sample was incubated with primary antibody (1/100 in PBS + 3% BSA + 0.1% Triton-X 100) for 24 hours at 4°C. ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab26 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Zhao M  et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun 9:583 (2018). IHC . Read more (PubMed: 29422534) »
  • Wang G  et al. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGFß1 and microRNA-34a-5p. Int J Mol Med 41:791-799 (2018). ICC/IF ; Human . Read more (PubMed: 29207015) »
See all 102 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Resulted in bright and vibrant staining.
Cells were fixed in 10% neutral buffered formalin.
Permeabilization: Triton X-100
Primary antibody: Rabbit polyclonal anti-calnexin (ab75801)
Blocking: 5% BSA in PBS
Counterstained with DAPI

Abcam user community

Verified customer

Submitted Dec 07 2018

Immunocytochemistry/ Immunofluorescence
I used this product by Abcam to stain the sensory epithelia of mouse inner ears.
Fixed samples were incubated overnight (4 c) with primary mouse anti myo7a antibody and on the next morning washed 3 times in PBS and then incubated for 2 hours in Abcam's goat anti mouse 488 antibody (ab150113), as well as DAPI and 647 phalloidin for 2 hours in room temp.
After mounting samples were imaged with Leica SP8 confocal microscope.
Staining shows specificity for hair cells.

Mr. Shahar Taiber

Verified customer

Submitted Oct 31 2018

Immunohistochemistry (Frozen sections)
I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)(ab150113) in kidney cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vivo studies

Dr. Shailendra Singh

Verified customer

Submitted Aug 17 2018

Alexa Fluor®
This antibody works perfectly. The performance is exactly the same of an Alexa Fluor.
I strongly recommend it.

Abcam user community

Verified customer

Submitted Nov 02 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
It worked perfectly at 1:500 dilution with a SALL4 (ab57577) mouse monoclonal. The intensity of the fluorescence was really great too, strongly recommend.

Dr. 煜欽 林

Verified customer

Submitted Oct 31 2013


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