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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (90)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

Key features and details

  • Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    ab150115 is specific to Mouse IgG.

    ab150115 has less than 47% cross-reactivity with rat IgG.

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 647

Associated products

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    • Goat Anti-Mouse IgG H&L (ab182017)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
  • Related Products

    • Normal Goat Serum (ab7481)
    • Anti-CD3 antibody [MEM-57] (ab8090)

Applications

Our Abpromise guarantee covers the use of ab150115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000 - 1/4000.

ab176103 - Mouse monoclonal IgG1 (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 647) (ab150115) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) and (ab16048, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 (red) goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab150077 Alexa Fluor® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei.

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    This data was developed using the unconjugated antibody (ab182017).

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182017).

  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (90)

    Publishing research using ab150115? Please let us know so that we can cite the reference in this datasheet.

    ab150115 has been referenced in 90 publications.

    • Coccia E  et al. SIVA-1 regulates apoptosis and synaptic function by modulating XIAP interaction with the death receptor antagonist FAIM-L. Cell Death Dis 11:82 (2020). PubMed: 32015347
    • Hirano T  et al. FARP1 boosts CDC42 activity from integrin avß5 signaling and correlates with poor prognosis of advanced gastric cancer. Oncogenesis 9:13 (2020). PubMed: 32029704
    • Mitobe J  et al. Superstructure formation by RodZ hexamers of Shigella sonnei maintains the rod shape of bacilli. PLoS One 15:e0228052 (2020). PubMed: 32053625
    • Zhao W  et al. NIPA2 regulates osteoblast function by modulating mitophagy in type 2 diabetes osteoporosis. Sci Rep 10:3078 (2020). PubMed: 32080264
    • Zhu Z  et al. Long non-coding RNA H19 promotes osteogenic differentiation of renal interstitial fibroblasts through Wnt-ß-catenin pathway. Mol Cell Biochem 470:145-155 (2020). PubMed: 32440841
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    It works well with Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    This Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) works well with Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) in mouse skin tissue.

    Abcam user community

    Verified customer

    Submitted Sep 17 2020

    AlexaFluor647 for Immunofluorescence

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    We used this AlexaFluor647 for IF with different primary antibodies including Gama Tubulin and Acetylated Tubulin for imaging. We used a 1:200 dilution and imaged via LSM 700 confocal microscopy. This dye was used in conjunction with some combination of DAPI, GFP or AlexFluor488(rabbit), and/or AlexaFluor594(rabbit). This dye was excellent, it stained the targets very well and showed little background noise.

    REVIEW30

    Abcam user community

    Verified customer

    Submitted Oct 30 2019

    An abreview for ab150115 - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    HeLa cells were cultured on cover slips and fixed with 4% paraformaldehyde in PBS (pH7.4) at room temperature for 10 min after exposed upon 10 uM choloroquine for 6 h. After washing three times with PBS, the cells were permeabilized with ice-cold methanol for 2.5 min. After three washes with PBS, the cells were incubated with blocking solution (5% BSA in PBS) for 1 h and then with primary antibody p62 overnight at 4ºC. The next day, the cells were washed five times for 5 min each time with PBS and then incubated with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor 647) for 1 to 2 h. The cells were washed four times with PBS for 5 min each time, and DAPI stained for 15 min. After three washes with PBS, the coverslips were mounted on slides using Fluoro-GEL (Electron Microscopy Sciences). Images were taken using Keyence BZ-X700.

    Abcam user community

    Verified customer

    Submitted Nov 01 2018

    Whole-mount immunofluorescence on mouse inner ear sensory epithelia

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Stained fixed sensory epithelia from p15 mice with M-anti-myosin7a overnight in 4 c, and then incubated the tissue for 2 hours in room temp with Abcam's goat anti mouse secondary 647 antibody (ab150115), as well as DAPI.
    Staining shows specificity for hair cells.

    Mr. Shahar Taiber

    Verified customer

    Submitted Oct 30 2018

    Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam ab150075 - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) in kidney cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vivo studies

    Dr. Shailendra Singh

    Verified customer

    Submitted Aug 17 2018

    ab150115 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) used with anti-GFP antibody ab38689.

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
    Antigen retrieval is optional in this staining. Blocking used was protein block. The left image is abcam's anti-GFP [6AT316] antibody (ab38689) used 1:200, for 12h at RT, with a competitors secondary antibody. On the right, is the same primary antibody, but this time used with abcam's Goat anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115) at 1:1000 dilution, for 3h at RT.

    Abcam user community

    Verified customer

    Submitted May 23 2014

    Question

    thank you for your mail, I chose ab76055. could you please confirm that the following secondary antibody AB 150115 is suitable for the antibody I'd like to buy?
    Thank you in advance for your attention
    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2012

    Answer

    Thank you for contacting us.

    I can confirm, that the Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (ab150115) would be suitable for detection of the Anti-E Cadherin antibody [M168] (ab76055).

    The ab150115 is covered by our six month guarantee for ICC/IF, ELISA, IHC-P and Flow Cyt applications.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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