We used this AlexaFluor647 for IF with different primary antibodies including Gama Tubulin and Acetylated Tubulin for imaging. We used a 1:200 dilution and imaged via LSM 700 confocal microscopy. This dye was used in conjunction with some combination of DAPI, GFP or AlexFluor488(rabbit), and/or AlexaFluor594(rabbit). This dye was excellent, it stained the targets very well and showed little background noise.
Abcam user community
Submitted Oct 30 2019
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