Product nameGoat Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Frmore details
mouse IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|WB||1/1000 - 1/4000.|
|Dot blot||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG
Observed band size: 28,55 kDa why is the actual band size different from the predicted?
Incubated with ab6790 for 60 min at room temperature.
Blocked for 30 min at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6790 has been referenced in 3 publications.
- Li X et al. Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells. Int J Environ Res Public Health 13:N/A (2016). IHC . PubMed: 26907321
- Anttila K et al. Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level. J Exp Biol 216:1183-90 (2013). PubMed: 23487268
- Huang HY et al. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn 238:232-40 (2009). PubMed: 19097054