Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, ELISA, WB, IHC-Pmore details
  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.10
    Preservative: 0.09% Sodium azide
    Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes

    Molar enzyme: antibody protein ratio is 1:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000. (Primary ELISA)
WB 1/5000 - 1/50000. (Chemiluminescent)
IHC-P 1/100 - 1/1000.

Images

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/5000 dilution
    Lanes 3-4 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/20000 dilution
    Lanes 5-6 : Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 minutes

References

This product has been referenced in:

  • Young DF  et al. The switch between acute and persistent paramyxovirus infection caused by single amino acid substitutions in the RNA polymerase P subunit. PLoS Pathog 15:e1007561 (2019). Read more (PubMed: 30742688) »
  • Huang LH  et al. Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B. Food Technol Biotechnol 57:105-112 (2019). Read more (PubMed: 31316282) »
See all 6 Publications for this product

Customer reviews and Q&As

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Tubulin

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Abreviews
Application
Western blot
5 ug of cell spermatocyte lysate with RIPA buffer
30 min skim milk blocking
Primary 1:2000 room temp for 1 hr
Secondary 1:2000 Room temp

Detection with Alkaline phosphatase substrate

Abcam user community

Verified customer

Submitted Jul 06 2016

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