Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, Dot blot, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
ELISA 1/7000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/2000.

Images

  • ab13918 staining RANK in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA/5% normal goat serum/0.1% saponin for 30 minutes at 22°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 9 hours at 4°C. An alkaline phosphatase-conjugated goat anti-mouse IgG H&L (ab7069) (1/300) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • McCarthy JJ  et al. Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development 138:3657-66 (2011). IHC-Fr . Read more (PubMed: 21828094) »
  • Laurentino SS  et al. Regucalcin is broadly expressed in male reproductive tissues and is a new androgen-target gene in mammalian testis. Reproduction 142:447-56 (2011). WB . Read more (PubMed: 21680783) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-CD68 (mouse) with Goat anti Mouse ALP (on human artery)

-Deparaffinize

-heat in citrate buffer 20min at 96°C

-H2O2 for 10min at room temperature

-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature

-incubate anti CD68 at 1/100 in PBS BSA Overnight at 4°C

-incubate with goat anti mouse ALP (1/300) for 45min at 37°C

-incubate slides in alkaline phosphatase substrate

-counterstain with hematoxylin, dehydrate and mount

Abcam user community

Verified customer

Submitted Mar 13 2014

Question
Answer

Thank you for your inquiry. I've included the link to the secondary antibody that we talked about on the phone.  This would work if your other antibody is raised in a mouse.  I've also included a link to some of our substrate kits. https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7069.html https://www.abcam.com/index.html?pageconfig=resource&rid=12886 https://www.abcam.com/index.html?pageconfig=resource&rid=12908 Here is the protocol from IHC world that talked about sequential double staining and a few other references: http://www.ihcworld.com/_protocols/general_IHC/immunoenzyme_double.htm http://www.ihcworld.com/_books/Dako_Handbook.pdf http://www.dako.com/08002_03aug09_ihc_guidebook_5th_edition_chapter_15.pdf This is the note I referenced about having bone sometimes come off the slide and a note about decalcification: http://www.histosearch.com/histonet/Jun99/BoneIHC.html http://www.scionpublishing.com/shop/ProductImages/Immunohistochemistry%20Chapter%204.pdf I hope this information helps.  Please contact us with any other questions.

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Answer

Thank you for your enquiry. As you mentioned in your e-mail, we currently have two Goat anti-mouse AP conjugated secondary antisera: ab7069 and ab6790. ab7069 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. ab6790 has not been prepared using a preabsorbtion step. For your needs in western blotting, I feel that either sera would be suitable. Usually if the application was IHC where endogenous IgG is likely to be present at higher levels then I think the preabsorbtion of sera may prove a useful purification step. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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