Overview

  • Product name
    Goat Anti-Mouse IgG H&L (AMCA)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    This antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Purified Mouse IgG

  • Conjugation
    AMCA. Ex: 350nm, Em: 445nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Conjugation notes
    This antibody has been conjugated to AMCA-X, SE [6-((7-Amino 4-methylcoumarin-3-acetyl)amino) hexanoic acid, succinimidyl ester].
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Alternative storage instructions:This product may be aliquoted and frozen at -70°C or below, without glycerol, avoiding repeated freezing and thawing.


    Blue fluorescent with absorption/emission wavelength at 353nm/442nm.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:
    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-Ki67, 1:100
    Rabbit anti-laminin alpha 1 (basement marker)
    Secondary antibodies:

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100
  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

References

ab47052 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Thank you for your email.

Unfortunately, we are not able to offer small sample sizes of our product due to operational reasons. However, we guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

And for untested species and applications, we offer the testing discount program, I had emailed you about recently.

For bulk orders of different products, please contact our customer service team atmailto:us.orders@abcam.comorby phone at 1-888-772-2226. For bulk orders of the same item (large quantities), please contact sales@abcam.com and my colleague will be able to assist you further.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab34766 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

Yes, ab47052 would bind to F(ab')2 since this antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins. The F(ab')2 fragment contains the light chains and part of the heavy chains.

The secondary antibody would be fine to use in flow cytometry and ELISA and we would guarantee it for these applications. I assume you have a plate reader for ELISA that covers the wavelength range?

The primary antibody has only been tested in flow cytometry, but not yet ELISA.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

Please let me know if I shall send you a testing discount code for trying ab34766 in ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your inquiry.

I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be detectable. Obviously the signal would however be stronger if the customer would have a filter letting pass 445nm as well. Please note however that the ab47052 is an anti mouse antibody.

The ab7086 coupled to FITC can also be used as emission maximum of FITC is at 519nm. Even though the spectrum is quite large, the emissionis very low under 500nm. (http://en.wikipedia.org/wiki/Fluorescein)I am therefore positive that those two fluorochromes can be used together without any significant interference.

Here is also a link to the our guide to fluorescent dyes: https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

The only thing I would like to draw your attention to, is that the ab47052 has not been crossabsorbed. This means we do not know whether this antibody would also recognize the rabbit antibodies. For a double staining it is therefore imperative that the customer makes this control (using only primary rabbit antibody and secondary anti mouse antibody to see whether there is any crossreaction). We unfortunately do not have another anti mouse antibody with a "blue" fluorochrome which would be already pre-absorbed. The ab7086 howeverhas been preabsorbed and is therefore perfectly fine for the double staining.

I hope this information is helpful. Please do not hesitate to get back to us, should you have any additional questions.

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Answer

Thank you for contacting us.

Unfortunately we do not havesecondary antibodies conjugated to Alexa 405 or Pacific Blue.
However, we have the following secondary antibodies, conjugated to AMCA, a blue fluorescence, Excitation/Emission wavelength= 344 nm/ 446 nm:

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA), https://www.abcam.com/ab47052
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AMCA), https://www.abcam.com/ab51438
Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AMCA), https://www.abcam.com/ab51452
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA), https://www.abcam.com/ab123435




Unfortunately, these products do not match your requirements andI hope this information is neverthelesshelpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for calling earlier today.

Below is a list to some secondary antibodies that may be useful to you:

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-594-pre-adsorbed-ab96881.html
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy5-pre-adsorbed-ab97037.html
https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-550-pre-adsorbed-ab98786.html



If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting us.

The information for AMCA is as follows:
Blue fluorescence
Excitation/Emission wavelength = 344 nm / 446 nm

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: https://www.abcam.com/Easylink

For the information regarding the discount codes please see below:

DISCOUNT CODE: xxx for ab34360
DISCOUNT CODE: xxx for ab16148
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab34360 and ab16148 in flow cytometry. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for flow cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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Answer

Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let me know how you would like to proceed.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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