Product nameGoat Anti-Mouse IgG H&L (APC) preadsorbed
See all IgG secondary antibodies
ab130782 reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3 and with the light chains of mouse IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat Anti-Mouse IgG H&L (Allophycocyanin) preadsorbed exhibits minimal cross reactivity with Human immunoglobulins.
Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG paraproteins
ConjugationAPC. Ex: 645nm, Em: 660nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, Sucrose
Concentration information loading...
Purification notesab130782 was purified by affinity chromatography on pooled Mouse IgG covalently linked to agarose.
General notesab130782 is cross absorbed against pooled Human sera and purified Human paraproteins.
Our Abpromise guarantee covers the use of ab130782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use 0.1µg for 106 cells.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab130782 has been referenced in 1 publication.
- Yuan X et al. Rhein enhances the cytotoxicity of effector lymphocytes in colon cancer under hypoxic conditions. Exp Ther Med 16:5350-5358 (2018). PubMed: 30542494