Overview

  • Product name
    Goat Anti-Mouse IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    mouse IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    The conjugate is expected to have between 5 and 10 moles of biotin per mole of IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
ICC Use at an assay dependent dilution. PubMed: 17980964
Dot blot Use at an assay dependent concentration.
ELISA 1/350000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/20000.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)

    IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6788)
    Ab6788 was used at dilution 1/500 with the primary antibody ab2866 in IHC-P. See the review on ab2866.

References

This product has been referenced in:
  • Lei X  et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). Read more (PubMed: 29693708) »
  • Chen H  et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). Read more (PubMed: 29694513) »

See all 13 Publications for this product

Publishing research using ab6788? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

The secondary antibody ab6788 gives no signal. We have compared it to an effective anti-mouse IgG secondary, also biotinylated.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team...

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Hi Abcam,

Do you mind helping with this enquiry below?

We have found another couple of secondary antibodies on your website that appear identical to AB6788 & AB6720 except for a different concentration.

They are: ab...

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Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of...

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I am interested in purchasing a secondary antibody for use in immunohistochemistry that can work with the following primary antibodies from your company (ab24590, ab7817, ab955, and ab89064). I have searched the catalog and found ab6728 and ab678...

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Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and i...

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Dear Sir/madam, Thank you very much for your kind attention. Secondary antibody that I used it is: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6788) I have used the same secondary antibody with another mouse monoclonal Antibody an...

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Thank you very much again for your reply. I appreciate the time you have spent in the laboratory and understand your concerns. As it appears to me your biotin-labeled secondary and the appropriate detection system are indeed working and it is regret...

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Dear Sir/madam, Thank you very much for your kind attention. These are the answers of all question: 1- No results, I mean that I obtain no bands at all. 2- a-The protein was denatured first. b- 5% milk c- 1:100 dilution ...

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Thank you very much for confirming these details. Before proceeding with this case, I would appreciate if you could confirm which detection system was used with the biotin-labeled secondary antibody ab6788: Have you used a HRP-conjugated anti-biotin...

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Hi, Could you please tell me the molecular weight of the antibody ab6788 in either daltons or grams (IgG is supposed to be 150,000 daltons in humans. But this one is from rabbit and has other modifications to it.) Also, your online information sheet sa...

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Thank you for your enquiry. Unfortunately, the molecular weight of this product is not specifically measured, but the molecular weight of non reduced IgG is approximately 150-160 kDa. The molecular weight of biotin is ~250 Daltons. Yes, there ...

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Thank you very much. Could you please let me know whether I can use PBS buffer as antibody diluent( my second query of ref:914295)

Thank you for getting back to me. Phosphate buffered saline can be used as an antibody diluent as an alternative to TBS for western blot analysis. However, with regards storage I would recommend that the antibodies are alituotted and frozen at -20o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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