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Hi, Could you please tell me the molecular weight of the antibody ab6788 in either daltons or grams (IgG is supposed to be 150,000 daltons in humans. But this one is from rabbit and has other modifications to it.) Also, your online information sheet says Biotin/protein ratio as 10 - 20. Does this mean there are 10 - 20 biotin molecules per Ab? Thank you
Asked on Oct 30 2009
Thank you for your enquiry. Unfortunately, the molecular weight of this product is not specifically measured, but the molecular weight of non reduced IgG is approximately 150-160 kDa. The molecular weight of biotin is ~250 Daltons. Yes, there are 10-20 molecules of Biotin conjugated to the IgG. The ratio of 10-20 is based on the combination ratio and typical results from our standard conjugation protocol. Function and purity of intact conjugate is confirmed by Immuno-electrophoresis (with anti biotin) and ELISA against mouse IgG using a HRP conjugated streptavidin as a secondary reagent. I hope this information is helpful. If there is anything else that I can help you with, please do not hesitate to contact me.
Answered on Oct 30 2009