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Do you mind helping with this enquiry below?
We have found another couple of secondary antibodies on your website that appear identical to AB6788 & AB6720 except for a different concentration.
They are: ab97049 Goat anti-rabbit IgG H&L (biotin)
and ab 97021 Goat polyclonal Secondary antibody to mouse IgG- H&L (Biotin)
What we are hoping to do is to enhance a western blot signal by adding a streptavidin step prior to applying a biotinylated secondary antibody. (This has been successfully done by colleagues in the past).
Would it be possible to get some advice as to which ones would be more suitable for our application?
Thanks for your help :D
Asked on Oct 15 2012
Thank you for contacting us and you interest in our products.
There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of conjugation. The ab6788 and ab6720 is estimated to have a conjugation ratio of 10-20 per IgG molecule whilst ab97049 and ab97021 have a ratio of 1:4.
I am not familiar with the amplification technique you intend to use and cannot therefore anticipate how these differences may alter the results.
If you could share a more detailed description of the procedure I may be able to advise in more detail.
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.
Answered on Oct 15 2012