Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7067)

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, WB, ELISA, Dot blotmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000 - 1/10000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA 1/1e+006.
Dot blot Use at an assay dependent dilution.

References

This product has been referenced in:

  • Liu L  et al. CP-31398 attenuates endometrial cancer cell invasion, metastasis and resistance to apoptosis by downregulating MDM2 expression. Int J Oncol 54:942-954 (2019). Read more (PubMed: 30628640) »
  • Lei X  et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). Read more (PubMed: 29693708) »
See all 5 Publications for this product

Publishing research using ab7067? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

What dilution do you suggest for ab7067 in IHC-P?

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Answer

Thank you for your recent telephone enquiry.

I am sorry it has taken a little time to confirm this information with the originator for you.

They suggest to use this antibody between 1:100 - 1:1000 in IHC-P. However, pleasethis is a guideline only and may require some optimization by the end user.

I hope this will be helpful to you, and that your preliminary experiment has gone well. If you have any further questions, please do not hesitate to contact us.

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Question

I am interested in obtaining some of your Biotinylated Goat anti Mouse IgG to use in a multiple step FACS staining for a dim surface antigen. I notice that you do not suggest the use of this Ab for FACS, is this because it does not work or because you have no data there?

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Answer

This antibody has never been tested specifically for FACS, therefore we can't guarantee results. However, since this antibody is a secondary, it will probably work well.

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