Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7067)

Submit a review Q&A (2)References (7)

Key features and details

  • Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, WB, ELISA, Dot blot

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, WB, ELISA, Dot blotmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/2000 - 1/10000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/1e+006.
Dot blot
Use at an assay dependent dilution.
Notes
ICC/IF
1/2000 - 1/10000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/1e+006.
Dot blot
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (7)

Publishing research using ab7067? Please let us know so that we can cite the reference in this datasheet.

ab7067 has been referenced in 7 publications.

  • Cui X  et al. Neuroprotective Effect of Optogenetics Varies With Distance From Channelrhodopsin-2 Expression in an Amyloid-ß-Injected Mouse Model of Alzheimer's Disease. Front Neurosci 14:583628 (2020). PubMed: 33162881
  • Liu L  et al. CP-31398 attenuates endometrial cancer cell invasion, metastasis and resistance to apoptosis by downregulating MDM2 expression. Int J Oncol 54:942-954 (2019). PubMed: 30628640
  • Wang KW  et al. Optogenetics-induced activation of glutamate receptors improves memory function in mice with Alzheimer's disease. Neural Regen Res 14:2147-2155 (2019). PubMed: 31397354
  • Lei X  et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). PubMed: 29693708
  • Lin L  et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). PubMed: 30066844
  • Simon-O'Brien E  et al. Etifoxine improves sensorimotor deficits and reduces glial activation, neuronal degeneration, and neuroinflammation in a rat model of traumatic brain injury. J Neuroinflammation 13:203 (2016). PubMed: 27565146
  • Laurentino SS  et al. Regucalcin is broadly expressed in male reproductive tissues and is a new androgen-target gene in mammalian testis. Reproduction 142:447-56 (2011). PubMed: 21680783

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

What dilution do you suggest for ab7067 in IHC-P?

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Answer

Thank you for your recent telephone enquiry.

I am sorry it has taken a little time to confirm this information with the originator for you.

They suggest to use this antibody between 1:100 - 1:1000 in IHC-P. However, pleasethis is a guideline only and may require some optimization by the end user.

I hope this will be helpful to you, and that your preliminary experiment has gone well. If you have any further questions, please do not hesitate to contact us.

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Question

I am interested in obtaining some of your Biotinylated Goat anti Mouse IgG to use in a multiple step FACS staining for a dim surface antigen. I notice that you do not suggest the use of this Ab for FACS, is this because it does not work or because you have no data there?

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Answer

This antibody has never been tested specifically for FACS, therefore we can't guarantee results. However, since this antibody is a secondary, it will probably work well.

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