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Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033)

Reviews (1)Q&A (1)References (3)

Key features and details

  • Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, IHC-P, ICC/IF, ELISA

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/200000. (Chemiluminescent).
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/10000 - 1/200000. (Primary).
Notes
WB
1/10000 - 1/200000. (Chemiluminescent).
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/10000 - 1/200000. (Primary).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab97033? Please let us know so that we can cite the reference in this datasheet.

ab97033 has been referenced in 3 publications.

  • Yan B  et al. Clinicopathological significance and prognostic implication of CD44 and its splice variants (v3 and v6) in colorectal cancer. Transl Cancer Res 9:1215-1224 (2020). PubMed: 35117466
  • Huang B  et al. Non-erythropoietic erythropoietin-derived peptide protects mice from systemic lupus erythematosus. J Cell Mol Med 22:3330-3339 (2018). PubMed: 29570934
  • Im H  et al. Digital diffraction detection of protein markers for avian influenza. Lab Chip 16:1340-5 (2016). PubMed: 26980325

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

mouse secondary antibody for western blot

 Excellent
Abreviews
abreview image
Application
Western blot
Used with Timp2 primary antibody ab1828
PAGE: 12%, reducing-denaturing
Blocking with 10% milk overnight
Secondary dilution 1:2000
Detection: ABC system
Band at around 24 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Henda Nabli

Verified customer

Submitted Oct 18 2013

Question

1) wie rein ist ab64255 (BSA?)? Ich moechte den AK radiomarkieren, biotin auch wichtig. 2) Konzentration/Volumen? "125ml at mg/ml" macht keinen Sinn.

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Answer

Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/ml, und weil es sich um ein ready-to-use-Produkt handelt, bekommen Sie tatsächlich 125 ml ! Für Ihre Versuchsvorhaben könnte sich dieser Antikörper daher vielleicht nicht unbedingt eignen - weil sich 125 ml doch eher schlecht verarbeiten lassen. Gerne möchte ich Ihnen zwei Alternativen nennen, die in weniger Volumen erhältlich sind (ab97021 und ab97033). Beide Antikörper sind biotinyliert, ab97033 ist zusätzlich noch prae-absorbiert, d.h. er reagiert nicht mit den auf dem Datenblatt angegebenen Spezies (wie Huhn, Rind, Ziege etc): Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-ab97021.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-preadsorbed-ab97033.html). Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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