Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033)

Reviews (1)Q&A (1)References (2)

Key features and details

  • Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, IHC-P, ICC/IF, ELISA

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab97033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/10000 - 1/200000. (Chemiluminescent).
    IHC-P 1/250 - 1/2500.
    ICC/IF 1/100 - 1/500.
    ELISA 1/10000 - 1/200000. (Primary).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab97033? Please let us know so that we can cite the reference in this datasheet.

    ab97033 has been referenced in 2 publications.

    • Huang B  et al. Non-erythropoietic erythropoietin-derived peptide protects mice from systemic lupus erythematosus. J Cell Mol Med 22:3330-3339 (2018). PubMed: 29570934
    • Im H  et al. Digital diffraction detection of protein markers for avian influenza. Lab Chip 16:1340-5 (2016). PubMed: 26980325

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    mouse secondary antibody for western blot

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Used with Timp2 primary antibody ab1828
    PAGE: 12%, reducing-denaturing
    Blocking with 10% milk overnight
    Secondary dilution 1:2000
    Detection: ABC system
    Band at around 24 kDa

    Mrs. Henda Nabli

    Verified customer

    Submitted Oct 18 2013

    Question

    1) wie rein ist ab64255 (BSA?)? Ich moechte den AK radiomarkieren, biotin auch wichtig. 2) Konzentration/Volumen? "125ml at mg/ml" macht keinen Sinn.

    Read More
    Answer

    Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/ml, und weil es sich um ein ready-to-use-Produkt handelt, bekommen Sie tatsächlich 125 ml ! Für Ihre Versuchsvorhaben könnte sich dieser Antikörper daher vielleicht nicht unbedingt eignen - weil sich 125 ml doch eher schlecht verarbeiten lassen. Gerne möchte ich Ihnen zwei Alternativen nennen, die in weniger Volumen erhältlich sind (ab97021 und ab97033). Beide Antikörper sind biotinyliert, ab97033 ist zusätzlich noch prae-absorbiert, d.h. er reagiert nicht mit den auf dem Datenblatt angegebenen Spezies (wie Huhn, Rind, Ziege etc): Click here (or use the following: https://www.abcam.com/index.html?datasheet=97021). Click here (or use the following: https://www.abcam.com/index.html?datasheet=97033). Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com