Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Chromeo™ 488)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    ab60313 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Immunogen

    Mouse IgG.

  • Conjugation

    Chromeo™ 488

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Conjugation notes

    Chromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets. Molar Extinction Coefficient: 73,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 48 % Excitation Wavelength Range: 470 to 500 nm Emission Wavelength Range: 515 to 530 nm
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000. Plate-based assays: 1/800 to 1/1000

Images

  • Chromeo 488 goat polyclonal to mouse IgG staining an alpha Tubulin mouse monoclonal antibody in HeLa cells by immunofluorescence.
  • ab60313, staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense with a DAPI nuclear counterstain.

References

This product has been referenced in:

  • Liu M  et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging. Nat Commun 8:15646 (2017). ICC/IF . Read more (PubMed: 28561031) »
  • Schleicher M  et al. Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility. Int J Biomater 2012:397813 (2012). Read more (PubMed: 22481939) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918



Since the isotype of this primary antibody is mouse IgG1, so you could select the isotype, target species, host species, format, specificity, conjugate using the drop-down menu.

We have different conjugates such as FITC, DyLight488, Chromeo 488, and here are a few suggestions:

FITC:

ab98692

ab97239

ab11588

You can find some further information about the the generation of our fluorochromes at this site:https://www.abcam.com/index.html?pageconfig=resource&rid=12948

DyLight488:

Affinity purified: ab96875, ab97015, ab96871

Pre-adsorbed: ab96879

Chromeo 488:

ab60313



If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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