Key features and details
- Goat Anti-Mouse IgG H&L (Chromeo™ 488)
- Conjugation: Chromeo™ 488
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF
Product nameGoat Anti-Mouse IgG H&L (Chromeo™ 488)
See all IgG secondary antibodies
Specificityab60313 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
Tested applicationsSuitable for: ICC/IFmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Conjugation notesChromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets. Molar Extinction Coefficient: 73,000 M-1cm
-1 (measured at Amax) Quantum Yield: ~ 48 % Excitation Wavelength Range: 470 to 500 nm Emission Wavelength Range: 515 to 530 nm
Our Abpromise guarantee covers the use of ab60313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/2000. Plate-based assays: 1/800 to 1/1000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab60313 has been referenced in 3 publications.
- Liu M et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging. Nat Commun 8:15646 (2017). ICC/IF . PubMed: 28561031
- Schleicher M et al. Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility. Int J Biomater 2012:397813 (2012). PubMed: 22481939
- Brobeil A et al. PTPIP51 is phosphorylated by Lyn and c-Src kinases lacking dephosphorylation by PTP1B in acute myeloid leukemia. Leuk Res 35:1367-75 (2011). ICC/IF . PubMed: 21513978