Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)

Reviews (2)Q&A (1)References (33)

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details

  • Conjugation

    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. PubMed: 24270184
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/2000 - 1/4000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)Wu JL et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015).

    (a–c) Immunofluorescent localization of histone H4 in early spermatids. (d) Ultrastructural localization of histone H4 in early spermatids. (e) Ultrastructural localization of histone H3 in intermediate spermatids. The secondary antibody used was Goat Anti-Mouse IgG H&L (Cy3 ® )preadsorbed (ab97035) at 1/200 dilution.

References

This product has been referenced in:

  • Losev Y  et al. Novel model of secreted human tau protein reveals the impact of the abnormal N-glycosylation of tau on its aggregation propensity. Sci Rep 9:2254 (2019). Read more (PubMed: 30783169) »
  • Yin S  et al. MicroRNA-130a regulated by HPV18 E6 promotes proliferation and invasion of cervical cancer cells by targeting TIMP2. Exp Ther Med 17:2837-2846 (2019). Read more (PubMed: 30906471) »
See all 33 Publications for this product

Publishing research using ab97035? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

excellent

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
We use this antibody together with the antibody ab2378 (Tryptase) in mastocytosis skin.
As working concentration we used a 1/100 dilution for 30minutes at room temperature.

Abcam user community

Verified customer

Submitted Feb 14 2018

Secondary Antibody for Immunocytochemisty with 2,2'-thiodiethanol (TDE) Mounting

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. The original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. Our results support this claim and the brightness seen in the red channel during image acquisition is considerable. Required exposure times/intensity settings for imaging are, consequently, very low compared to similar fluorophores.
The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (594 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.

Dr. Sam Nowitzki

Verified customer

Submitted Jul 09 2015

Question

Hi, Thanks for your message and providing me options. I'd like to try the CY 3 (ab97035) in WB and take part in the testing scheme. I'd be grateful if you can send me the discount code. Kind regards

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Answer

Thank you for your reply.
I'm pleased to hear you would like to participate in the testing discount scheme with the Goat anti-Mouse IgG H&L (Cy3 ®) secondary antibody (ab97035). The discount code is as follows:
Discount code: xxxxxxxx
Expiration date: 17th October 2012
In order to activate this code, please submit an Abreview for ab97035 in Western blotting. Include the code above in the "Additional comments" section of the Abreview form. It does not matter whether the review is positive or negative, we just want to know your results. You can then use the code in your next order with us to redeem your free secondary antibody (or the equivalent value of ab97035 off any product in the catalogue).
More information on this scheme can be found from the following link:
www.abcam.com/collaborationdiscount
I hope this has been of help. If you require any further information please do not hesitate to contact us again.

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