Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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Tested applications
Suitable for: ICC/IFmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details -
Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.80
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50 - 1/500.
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| Notes |
|---|
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ICC/IF
1/50 - 1/500. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)Wu JL et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015).(a–c) Immunofluorescent localization of histone H4 in early spermatids. (d) Ultrastructural localization of histone H4 in early spermatids. (e) Ultrastructural localization of histone H3 in intermediate spermatids. The secondary antibody used was Goat Anti-Mouse IgG H&L (Cy3 ® )preadsorbed (ab97035) at 1/200 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (87)
ab97035 has been referenced in 87 publications.
- Puttipanyalears C et al. Quantitative STAU2 measurement in lymphocytes for breast cancer risk assessment. Sci Rep 11:915 (2021). PubMed: 33441653
- Cao S et al. Comparisons of neuroinflammation, microglial activation, and degeneration of the locus coeruleus-norepinephrine system in APP/PS1 and aging mice. J Neuroinflammation 18:10 (2021). PubMed: 33407625
- Rajbhandari AK et al. A Basomedial Amygdala to Intercalated Cells Microcircuit Expressing PACAP and Its Receptor PAC1 Regulates Contextual Fear. J Neurosci 41:3446-3461 (2021). PubMed: 33637560
- Atiakshin D et al. Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis. Histochem Cell Biol 155:561-580 (2021). PubMed: 33492488
- Legartová S et al. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells 10:N/A (2021). PubMed: 33535591