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I want to do a triple antibody stain using two primary antibodies produced in mice with different isotypes (e.g. IgG1 and IgG2b). I purchased the goat polyclonal secondary antibody to mouse IgG - H&L (Cy5), pre-adsorbed (ab6563) and I was wondering if this antibody is specific for one subclass of IgG or if it detects multiple IgG subclasses.
Asked on Feb 21 2013
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This antibody detects all mouse IgG, so it should work with both your IgG1 and IgG2 antibodies.
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Answered on Feb 21 2013