Product nameGoat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
See all IgG secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5.5 ®), pre-adsorbed
Tested applicationsSuitable for: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopymore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Mouse IgG (purified).
ConjugationCy5.5 ®. Ex: 678nm, Em: 703nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
Conjugation notesCy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 3.7 moles Cy5.5 per mole of Goat IgG
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Our Abpromise guarantee covers the use of ab6947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|ELISA||1/10000 - 1/50000.|
|Flow Cyt||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6947 has been referenced in 6 publications.
- Mendez-Bermudez A et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
- Holloway PM et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
- Wilhelm T et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
- Técher H et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
- Letessier A et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320