Overview

  • Product name
    Goat Anti-Mouse IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/1000 - 1/2000.

Images

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488) (ab96871) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:
  • Langhauser F  et al. A diseasome cluster-based drug repurposing of soluble guanylate cyclase activators from smooth muscle relaxation to direct neuroprotection. NPJ Syst Biol Appl 4:8 (2018). Read more (PubMed: 29423274) »
  • Gui Z  et al. Oridonin inhibition and miR-200b-3p/ZEB1 axis in human pancreatic cancer. Int J Oncol 50:111-120 (2017). Read more (PubMed: 27878247) »
See all 9 Publications for this product

Customer reviews and Q&As

11-12 of 12 Abreviews or Q&A

Answer

My colleague forwarded your message about testing an Abcam IGF2 antibody to me.

As he mentioned, if western blotting and IHC are listed as applications on the datasheet, and rat and mouse are listed, then the antibody will be guaranteed for those applications and species and a replacement or credit or refund is available in the event it fails. If not, it is not guaranteed.

For untested applications or species, we do not have samples, but we offer the testing discount he mentioned.

The procedure is as follows: I first issue a discount code to you. Then, your lab purchases the antibody, tests it, and sends us your results in the form of a review (www.abcam.com/abreviews has some information about this). Once we have your review, your testing discount code becomes active, regardless of whether the results are positive or negative.

The discount is a credit that can be applied to a subsequent order, good for one free primary antibody of your choice. I will be happy to issue you a code before you purchase. I just need the antibody catalogue number, and I will send you a code, which you include in the notes section of the review form.

For some examples of customer reviews, please see the datasheet for anti-IGF2 ab9574:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=9574).

Regarding a control peptide, this is available for some antibodies, but not all. If so, it will be a separate item for purchase.

I look forward to your reply.

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Answer

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96873). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96881). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=97022). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102884). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102885). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102923). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102924). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102925). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=97050). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96899). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96885). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96897). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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11-12 of 12 Abreviews or Q&A

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