Overview

  • Product name
    Goat Anti-Mouse IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/1000 - 1/2000.

Images

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488) (ab96871) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:
  • Langhauser F  et al. A diseasome cluster-based drug repurposing of soluble guanylate cyclase activators from smooth muscle relaxation to direct neuroprotection. NPJ Syst Biol Appl 4:8 (2018). Read more (PubMed: 29423274) »
  • Gui Z  et al. Oridonin inhibition and miR-200b-3p/ZEB1 axis in human pancreatic cancer. Int J Oncol 50:111-120 (2017). Read more (PubMed: 27878247) »
See all 9 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunocytochemistry
1. Fixation: 95% ethanol for 10 min
2. Washing: 1x PBS 3 min
3. Blocking: 1% BSA in PBST for 30 min
4. Primary antibody incubation (anti-vimentin AB28028): in 1% BSA in PBST for overnight (4°C)
5. Secondary antibody incubation (goat anti-mouse ab): in 1% BSA in PBST for 30 min
6. Mounting: Fluoroshield™

Dr. Egi Kardia

Verified customer

Submitted Sep 11 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab4648 staining GFAP in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 in PBS and blocked with 10% Serum for 40 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in TBS) for 24 hours at 4°C. ab96871 was used as the secondary antibody at a dilution of 1/200. ( Green = GFAP immunostained astrocytes, Blue = DAPI stained nuclei.)

Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
1. Cells are Fixed in 2% Paraformaldehyde, (PFA) - 45min at Room Temperature.
2. Permeabilized with 0.5% TritonX -100 in TBS for 7min - 3washes
3. Proteins are blocked with 2% BSA in TBS with 0.1% TritonX -100 for 2 hours.
4. The concentration of DyLight 488 used for this experiment is 1:500 and incubated for 2 hours.

Abcam user community

Verified customer

Submitted Aug 19 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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