Question (57944) | Goat Anti-Mouse IgG H&L (DyLight® 488) (ab96871)

Go to datasheet (ab96871)

Question

Dear tech Support

I have another query from a customer. I did send her the data sheets and concentration supplied. On the data sheet the suggested dilution is given, but she still has the following question. Please help. The antibodies in question:
AB96875
AB96871
AB96877
AB96873

Thank you for all your help

Regards

I need to know the concentration of antibody needed per reaction? So what volume of the given stock would I need to use in one experiment?
Best regards

Answer

Thank you for contacting us and your interest in our products.

The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in these experiments.

If the customer could please answer the following questions I can help in their request:

1. What application are you hoping to use these antibodies in?

2. Using your protocol, what volume of diluted antibody do you usually apply?

I look forward to receiving your reply.

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