Question (58264) | Goat Anti-Mouse IgG H&L (DyLight® 488) (ab96871)

Go to datasheet (ab96871)

Question

Dear xxxxxx,

Below is the reply from my customer:

I wish to use the antibodies in a co-localization experiment. We have primary antibodies against specific Plasmodium falciparum proteins which localize to two different parasite organelles. The fluorescent secondary antibodies will then allow these organelles to be visualized.

We have not performed an experiment like this in the lab before and we will have to develop a protocol. This is the reason why I was wondering what the recommended protocol would be for the DyLight secondary antibodies.

I'm on a very strict budget and I'm looking for the most cost effective way to perform this experiment. In comparison, the Alexa Fluor secondary antibodies have a working concentration of 1ug/ml, with a supplied concentration of 2mg/ml. Do the DyLight secondary antibodies, with a supplied concentration of 0.5mg/ml, have a lower working concentration than that of the Alexa Fluor?

Thank you for your help

Regards

Answer

Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these conditions will be required by the end used and their particular experimental conditions. This will be the case with any secondary antibody. This equates to a final concentration of 1 to 10 ug/mL.

How many secondary antibodies are you looking to purchase? It may be that I can arrange for a discount for you?

I hope this information has been of help. I look forward to receiving your reply.

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