ICC on LNCaP cells

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Abreviews
Application
Immunocytochemistry/ Immunofluorescence
1. Cells are Fixed in 2% Paraformaldehyde, (PFA) - 45min at Room Temperature.
2. Permeabilized with 0.5% TritonX -100 in TBS for 7min - 3washes
3. Proteins are blocked with 2% BSA in TBS with 0.1% TritonX -100 for 2 hours.
4. The concentration of DyLight 488 used for this experiment is 1:500 and incubated for 2 hours.

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Submitted Aug 19 2015

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