Immunocytochemistry (primary skin fibroblast cells)

Excellent
Abreviews
Application
Immunocytochemistry
1. Fixation: 95% ethanol for 10 min
2. Washing: 1x PBS 3 min
3. Blocking: 1% BSA in PBST for 30 min
4. Primary antibody incubation (anti-vimentin AB28028): in 1% BSA in PBST for overnight (4°C)
5. Secondary antibody incubation (goat anti-mouse ab): in 1% BSA in PBST for 30 min
6. Mounting: Fluoroshield™

Dr. Egi Kardia

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Submitted Sep 11 2018

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