Overview

  • Product name
    Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/1000 - 1/2000.

Images

  • ab85137 staining S100 in a human melanoma cell line by Flow Cytometry. The cells were harvested using EDTA and washed in PBS. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at room temperature. A DyLight® 488-conjugated goat anti-mouse IgG H&L (ab96879) (1/100) was used as the secondary antibody.

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488, pre-adsorbed) (ab96879) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

  • ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). Read more (PubMed: 27924000) »
  • Zhao X  et al. Effects of 17-allylamino-17-demethoxygeldanamycin on the induction of apoptosis and cell cycle arrest in HCT-116 cells. Oncol Lett 14:2177-2185 (2017). Read more (PubMed: 28789442) »
See all 22 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

I performed a BLAST search with the general term "fish" and only the match below.

poly [ADP-ribose] polymerase 1-like [Takifugu rubripes] 91%

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

According to my records, ab139923 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.



Looking at our correspondence, it appears that we are awaiting protocol details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.



If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.



We wish you the best of luck with your research and look forward to a reply.

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Answer

Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are

https://www.abcam.com/Nanog-antibody-ab70482.html

https://www.abcam.com/Nanog-antibody-ab90640.html

7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

11. We do not sell this chemical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us and asking this question. It does not seem stupid to me at all!

You are correct, you need a secondary antibody that is conjugated to a fluorescent molecule (or an enzyme, followed by the enzyme's substrate to get some color) in order to see where ab9489 is sticking to CD31. The one you picked out, ab96879, is a good choice, assuming you have a microscope that detects fluorescence and has the correct filter for that fluorochrome, DyLight® 488. (It very likely does).

I hope this helps. If you have any other questions, please let us know.

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Answer

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I can confirm the Safety Data Sheets can be found on the online datasheets. Near the top right hand side of the datasheet, there are two 'buttons', one for Datasheet PDF and the one underneath says SDS. Click on the SDS button to link to the document.

I hope this will be helpful. If you have any difficulties finding these, or if you have any further questions, please do not hesitate to contact me.

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Answer

Thank you for contacting us.
I am sorry to hear that the antibodies are not working as expected.
As we discussed over the phone, if you please could try the no primary control, this would help us a lot in determining whether the problem comes from the secondary antibody alone, or if there are issues with the primary antibody and isotype control as well.
Please let me know your results.
I look forward to hear back from you and assist you further in resolving his problem.

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Answer

Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC-P, ICC/IF, Flow Cyt, and WB.

It is therefore tested and guaranteed for western blot and immunohistochemistry on paraffin embedded sections. I am sorry I am not sure what you mean by IHC both FL - Non FL, could you clarify?

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Answer

Thank you for contacting us.

The fluorochromes which are sold under the brand names Alexa, DyLight or Chromeo are designed to ensure an optimal performance and thus are chemically almost identical. As you may have noticed the advantagesfor these include excellent photostability and bright fluorescence (see also flyer attached). So from this point of view, there is not much of a difference between the DyLights or Chromeos.

Therefore, you may want to make your decision based on other factors:

We offer DyLight conjugated antibodies in the wavelengths 488nm, 550nm, 594nm and 650nm (based on the excitation maxima). These fluorochromes are suitable for most settings. We have Chromeo conjugated antibodies in comparable wavelengths available (488nm, 546nm, 642nm), but also for 494nm and 505nm which are somewhere in between. So if you wish to stain your target in ones of these colours (and your microscope has the correct filter settings), I would recommend the Chromeos.

On the other hand, our DyLight antibody range contains a wide variety of antibody formats, such as antibodies against H&L chain (e.g. ab96879), against the Fc part (e.g. ab98736) or F(ab')2 fragments (e.g. ab98739). Also, within our DyLight antibody range we have a number of secondaries available which have been pre-absorbed against other species to minimise potential cross-reactivity (e.g. ab96879). These advantages might be relevant for you depending on the sample type and species and the primary antibody.

Regarding the resistance to photobleaching, I would slightly prefer the DyLight conjugated antibodies, asaccording to customer feedback they seem to perform excellent. However, this is my opinion only and merelybased on our sales volume for the DyLights; we do not have any further data to confirm this.

I hope this information helps. Please do not hesitate to contact us if you need anything further or if I could help you finding the most suitable secondary for your experiments.

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1-10 of 13 Abreviews or Q&A

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