Goat Anti-Mouse IgG H&L (DyLight® 550) (ab96872)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 550)
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Goat Anti-Mouse IgG H&L (DyLight® 550)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 550 replaces DyLight® 549. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab96872 has been referenced in 6 publications.
- Chan DKH et al. Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9. STAR Protoc 4:101978 (2023). PubMed: 36598849
- Olejnik A et al. Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes. Int J Mol Sci 23:N/A (2022). PubMed: 36555091
- Olejnik A et al. The Klotho protein supports redox balance and metabolic functions of cardiomyocytes during ischemia/reperfusion injury. Cardiol J N/A:N/A (2021). PubMed: 34967938
- Hoyt AK et al. PRP-1 significantly decreases the ALDHhigh cancer stem cell population and regulates the aberrant Wnt/ß-catenin pathway in human chondrosarcoma JJ012 cells. Oncol Rep 42:103-114 (2019). PubMed: 31180539
- Li Q et al. Fbxl4 Serves as a Clock Output Molecule that Regulates Sleep through Promotion of Rhythmic Degradation of the GABAA Receptor. Curr Biol 27:3616-3625.e5 (2017). PubMed: 29174887
- Tang H & Han M Fatty Acids Regulate Germline Sex Determination through ACS-4-Dependent Myristoylation. Cell 169:457-469.e13 (2017). PubMed: 28431246