Product nameGoat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
See all IgG secondary antibodies
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationDyLight® 550. Ex: 562nm, Em: 576nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
General notesDyLight® 550 replaces DyLight® 549.
Our Abpromise guarantee covers the use of ab96880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
This product has been referenced in:
- Klepinin A et al. The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins. J Bioenerg Biomembr N/A:N/A (2018). Read more (PubMed: 29998379) »
- Wagener KC et al. Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology. Antioxid Redox Signal 25:41-58 (2016). ICC/IF . Read more (PubMed: 27059697) »