Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details

  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    DyLight® 550 replaces DyLight® 549.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)
    Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Klepinin A  et al. The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins. J Bioenerg Biomembr N/A:N/A (2018). Read more (PubMed: 29998379) »
  • Wagener KC  et al. Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology. Antioxid Redox Signal 25:41-58 (2016). ICC/IF . Read more (PubMed: 27059697) »
See all 7 Publications for this product

Publishing research using ab96880? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Could you confirm if any of the dylight dyes are brighter than other dyes available for around 568 nm (eg alexa 568 nm)

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Answer

Thank you for your recent telephone enquiry. I am forwarding a link to our Dyelight information page from the Abcam website: https://www.abcam.com/index.html? pageconfig=resource&rid=12934#2 I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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