Goat Anti-Mouse IgG H&L (DyLight® 594) (ab96873)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (DyLight® 594)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation
    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Chang KW  et al. Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development. BMC Genomics 19:425 (2018). Read more (PubMed: 29859049) »
  • Zhang H  et al. The protection role of Atg16l1 in CD11c+dendritic cells in murine colitis. Immunobiology 222:831-841 (2017). Read more (PubMed: 28390705) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab76055 staining E-Cadherin in mouse lung tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 in PBS and blocked with 1% BSA for 40 minutes at 25°C. Samples were incubated with primary antibody (1/200 in TBS) for 24 hours at 4°C. ab96873 was used as the secondary antibody at a dilution of 1/200. ( Red =E-Cadherin, Blue = DAPI stained nuclei.)
Username

Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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