Overview

  • Product name

    Goat Anti-Mouse IgG H&L (DyLight® 594)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Wan XQ  et al. SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. Int J Mol Med 43:371-381 (2019). Read more (PubMed: 30387808) »
  • Xu G  et al. Restraint Stress Induced Hyperpermeability and Damage of the Blood-Brain Barrier in the Amygdala of Adult Rats. Front Mol Neurosci 12:32 (2019). Read more (PubMed: 30814927) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab76055 staining E-Cadherin in mouse lung tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 in PBS and blocked with 1% BSA for 40 minutes at 25°C. Samples were incubated with primary antibody (1/200 in TBS) for 24 hours at 4°C. ab96873 was used as the secondary antibody at a dilution of 1/200. ( Red =E-Cadherin, Blue = DAPI stained nuclei.)

Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Answer

Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these conditions will be required by the end used and their particular experimental conditions. This will be the case with any secondary antibody. This equates to a final concentration of 1 to 10 ug/mL.

How many secondary antibodies are you looking to purchase? It may be that I can arrange for a discount for you?

I hope this information has been of help. I look forward to receiving your reply.

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