Overview

  • Product name

    Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • ab96881 was used at dilution 1:50 for 30 min for IHC analysis of FFPE human kidney. The primary antibody was a Mouse anti Human CD31 RTU for 30 min. High pH HIER was used as the antigen retrieval. A DAPI nuclear counterstain was also used. Right image: negative control

    This image is courtesy of Benjamin Kriederman (HistoTox Labs, Inc.)

References

This product has been referenced in:

  • Fang CL  et al. Liposome-Encapsulated Baicalein Suppressed Lipogenesis and Extracellular Matrix Formation in Hs68 Human Dermal Fibroblasts. Front Pharmacol 9:155 (2018). Read more (PubMed: 29559910) »
  • Wang Z  et al. Atf3 deficiency promotes genome instability and spontaneous tumorigenesis in mice. Oncogene 37:18-27 (2018). Read more (PubMed: 28869597) »
See all 8 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96873). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96881). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96871). Click here (or use the following: https://www.abcam.com/index.html?datasheet=97022). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102884). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102885). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/index.html?datasheet=102923). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102924). Click here (or use the following: https://www.abcam.com/index.html?datasheet=102925). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/index.html?datasheet=97050). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96883). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96899). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96885). Click here (or use the following: https://www.abcam.com/index.html?datasheet=96897). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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