Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed
    See all IgG secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 650), pre-adsorbed
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Smith SE  et al. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro. J Virol 87:12957-66 (2013). Flow Cyt . Read more (PubMed: 24067955) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
ab96882 is used to detect mouse transfferin receptor (red channel), After primary anibody incubation, ab96882 is incubated at room temperature in 2%BSA/PBS for 1h. Confocal imaging showing nice punctate staining on live cells.

Ms. Ya Zhou

Verified customer

Submitted Jan 29 2014

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to investigate this particular case further for you, and also obtain more information for our quality records. In order to proceed with this, there are a few things I would like to clarify to better understand and solve the problems experienced with this antibody.

-In which species is the primary antibody raised?

-Have you tested different dilutions combinations?

-Was a positive control used to assess the antibodies performance?

-If fixation was performed with formalin in paraffin embedded sections, antigen retrieval may be needed. Was the antigen retrieved at all after fixation?

-It would be very useful to test the secondary antibody with a different primary, as well as performing detecting the primary with a different secondary, to help to find out the source of the problem.

-Incubating the primary at 4C overnight could also give better staining results.

I would appreciate if you could provide further details, and encourage you to perform the mentioned suggestions in order to improve the results.

In any case, as the product is still under our guarantee, please don’t hesitate to contact us if the results do not improve.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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