Overview

  • Product name
    Goat Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details
  • Immunogen

    mouse whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/500 - 1/2500. PubMed: 17989178
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
ICC/IF 1/1000 - 1/5000. (PMID16006199)
ELISA 1/10000 - 1/50000.
WB 1/10000. PubMed: 17217624

Images

  • ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

    See Abreview

  • Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
  • Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
  • Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

References

This product has been referenced in:
  • Özdemir S  et al. Imidacloprid exposure cause the histopathological changes, activation of TNF-a, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpioL.). Toxicol Rep 5:125-133 (2018). Read more (PubMed: 29321977) »
  • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »
See all 114 Publications for this product

Customer reviews and Q&As

1-10 of 19 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam Attached please find the two MSDS forms you requested. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

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Question
Answer

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that this took longer than expected, but I hope that this information will be useful. Please let me know if you have any questions and I'll be happy to help.

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Question
Answer

Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happen within the next couple of days.

Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

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Answer

Thank you for the follow-up. The article may help, but I think an easy work-around is to try the red channel instead of green, if that is an option. Autofluorescence is generally less of an issue in the red, but you will need a red secondary, such as ab96881 (equivalent to Texas Red or Alexa Fluor 594):

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96881).

You will want to check that unstained section to see if the background autofluorescence is a problem in that channel though.

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Question
Answer

Thank you for your call today and for your questions. Please see below for information about our testing discount program, and let me know if you have any further questions about these antibodies or our IHC protocols.

Sinceab63983 has not been tested frozen tissue sections (IHC-Fr), I can offer a discount off a future purchase if you buy ab63983 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. The discount would be worth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab63983 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab63983 so please wait for my reply before ordering.

2. Purchase ab63983 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63983 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your inquiry.


We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


I would reccomend using ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) instead.


https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6785.html


I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse staining, which will give you information on how to prevent the high background. One popular method is to directly conjugate your primary antibody to a fluorescent tag. We do sell a number of these kits, please see the link below: https://www.abcam.com/easylink If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting us. The x-ray film may be able to detect fluorescence emission, but the problem is that the FITC would need to be excited with light to emit light, and that would presumably expose all of the film to the excitation light. Luminescence from ECL, on the other hand, is a chemical reaction, and does not require an excitation light. Unfortunately, I think you will need to purchase an HRP-conjugated secondary antibody, unless you have access to a fluorescent detection system. If the secondary antibody ab6785 was purchased within the past week, we will be able to take it back, as long as it is unopened. There will also be a 20% re-stocking fee.

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Answer

Thank you for contacting Abcam. The product ab6785, the goat polyclonal secondary antibody to mouse IgG - H&L is conjugated with FITC. The only way to detect FITC is with a laser that has the ability to Excite at 494nm and a filter that can detect Emission at 518nm. I do not know of any other way that you can detect FITC. The antibody ab97023, goat polyclonal secondary antibody to mouse IgG - H&L (HRP), would be an appropriate secondary to use with conventional western blot detection systems. If there is anything I can help you with, please let me know. .

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1-10 of 19 Abreviews or Q&A

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