Goat Anti-Mouse IgG H&L (FITC) (ab6785)

Reviews (1)Q&A (19)References (291)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
  • Immunocytochemistry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

Key features and details

  • Goat Anti-Mouse IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details

  • Immunogen

    mouse whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
1/1000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500. PubMed: 17989178
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
ICC/IF (1)
1/1000 - 1/5000. (PMID16006199)
ELISA
1/10000 - 1/50000.
WB
1/10000. PubMed: 17217624
Notes
IHC (PFA fixed)
1/1000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500. PubMed: 17989178
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
ICC/IF
1/1000 - 1/5000. (PMID16006199)
ELISA
1/10000 - 1/50000.
WB
1/10000. PubMed: 17217624

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.

    ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
  • Immunocytochemistry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunocytochemistry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (291)

Publishing research using ab6785? Please let us know so that we can cite the reference in this datasheet.

ab6785 has been referenced in 291 publications.

  • Makita E  et al. Autoantibodies to BPAG1e Trigger Experimental Bullous Pemphigoid in Mice. J Invest Dermatol 141:1167-1176.e3 (2021). PubMed: 33069726
  • Glorieux C  et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol 38:101780 (2021). PubMed: 33171331
  • Zhou D  et al. Acarbose ameliorates spontaneous type-2 diabetes in db/db mice by inhibiting PDX-1 methylation. Mol Med Rep 23:N/A (2021). PubMed: 33236139
  • Ding Y  et al. Anti-NMDAR encephalitis induced in mice by active immunization with a peptide from the amino-terminal domain of the GluN1 subunit. J Neuroinflammation 18:53 (2021). PubMed: 33612107
  • Vishnyakova P  et al. Effects of platelet-rich plasma on mesenchymal stem cells isolated from rat uterus. PeerJ 8:e10415 (2020). PubMed: 33335809
View all Publications for this product

Customer reviews and Q&As

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1-10 of 20 Abreviews or Q&A

Secondary antibody gives great fluorescent signals

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
We have used Vimentin ab for IF on human fibroblasts, 1:1000 dilution in FBS 5% in PBST for 60 min. Secondary ab, Goat anti-mouse FITC 1:500 for 30 min RT.

Mr. Michael Kirr

Verified customer

Submitted Feb 26 2021

Question

Can you please send me an MSDS for ab8879 and ab6785?

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Answer

Thank you for contacting Abcam Attached please find the two MSDS forms you requested. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

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Question

Needs a streptavidin-FITC protein.

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Answer

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that this took longer than expected, but I hope that this information will be useful. Please let me know if you have any questions and I'll be happy to help.

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Question

Needs a streptavidin-FITC protein.

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Answer

Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happen within the next couple of days.

Please let me know if you have any questions or if there is anything else that we can do for you.

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Question

We’ve packaged the other antibody up and are awaiting FedEx pickup.

Thanks for sending the correct antibody asap.

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Answer

Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

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Question

Thanks. It is the autofluorescence , causing the problem. I have looked
at the section without antibodies & it fluoresces. I am reading the
articles, hope will come over the problem.

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Answer

Thank you for the follow-up. The article may help, but I think an easy work-around is to try the red channel instead of green, if that is an option. Autofluorescence is generally less of an issue in the red, but you will need a red secondary, such as ab96881 (equivalent to Texas Red or Alexa Fluor 594):

Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-594-preadsorbed-ab96881.html).

You will want to check that unstained section to see if the background autofluorescence is a problem in that channel though.

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Question

Interested in using ab63983 on frozen mouse brain tissue sections.

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Answer

Thank you for your call today and for your questions. Please see below for information about our testing discount program, and let me know if you have any further questions about these antibodies or our IHC protocols.

Sinceab63983 has not been tested frozen tissue sections (IHC-Fr), I can offer a discount off a future purchase if you buy ab63983 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. The discount would be worth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab63983 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab63983 so please wait for my reply before ordering.

2. Purchase ab63983 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63983 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

Can Goat polyclonal Secondary Antibody to Mouse IgA - alpha chain (FITC) (ab97234) be used as seondary antibody for "Anti-Francisella tularensis LPS antibody [FB11] (ab2033) in indirect immunofluorescence assay?

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Answer

Thank you for your inquiry.


We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


I would reccomend using ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) instead.


https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6785.html


I hope this information helps. Please contact us with any other questions.

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Question

I want to do an IF application on mouse tissue using a mouse anti-Helicobacter pylori antibody as primary antibody and a goat polyclonal secondary antibody to mouse IgG (FITC conjugated). Do you think it should work? I would like to thank you in advance With kind regards

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Answer

Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse staining, which will give you information on how to prevent the high background. One popular method is to directly conjugate your primary antibody to a fluorescent tag. We do sell a number of these kits, please see the link below: https://www.abcam.com/easylink If there is anything else I can help you with, please let me know.

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Question

I am writing regarding the product with catalogue number ab6785, We are planning to run a western blotting experiment using ABcam primary antibodies and also using secondary antibody with the cat number ab6785. However we thought this secondary antibody is conjugated with hordish perexidase, however we were surprised to have Diaminotriazinylaminofluorescein conjucated with the secondary antibody. Now we are asking how to detect the fluorescence that emitted from the antibody, can we use the Xray detection (the only detection system that available to us) or do we have to buy a new detection system???? What are the available options for helping us. We really need the answer ASAP

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Answer

Thank you for contacting us. The x-ray film may be able to detect fluorescence emission, but the problem is that the FITC would need to be excited with light to emit light, and that would presumably expose all of the film to the excitation light. Luminescence from ECL, on the other hand, is a chemical reaction, and does not require an excitation light. Unfortunately, I think you will need to purchase an HRP-conjugated secondary antibody, unless you have access to a fluorescent detection system. If the secondary antibody ab6785 was purchased within the past week, we will be able to take it back, as long as it is unopened. There will also be a 20% re-stocking fee.

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1-10 of 20 Abreviews or Q&A

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