Overview

  • Product name
    Goat Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details
  • Immunogen

    mouse whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/500 - 1/2500. PubMed: 17989178
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
ICC/IF 1/1000 - 1/5000. (PMID16006199)
ELISA 1/10000 - 1/50000.
WB 1/10000. PubMed: 17217624

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.

    ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
  • Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

References

This product has been referenced in:
  • Özdemir S  et al. Imidacloprid exposure cause the histopathological changes, activation of TNF-a, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpioL.). Toxicol Rep 5:125-133 (2018). Read more (PubMed: 29321977) »
  • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »

See all 105 Publications for this product

Publishing research using ab6785? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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19 reviews or Q&A

10
Votes: Highest First

Can you please send me an MSDS for ab8879 and ab6785?

Thank you for contacting Abcam Attached please find the two MSDS forms you requested. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

Needs a streptavidin-FITC protein.

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that thi...

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Needs a streptavidin-FITC protein.

Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happ...

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We’ve packaged the other antibody up and are awaiting FedEx pickup.

Thanks for sending the correct antibody asap.

Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

Thanks. It is the autofluorescence , causing the problem. I have looked
at the section without antibodies & it fluoresces. I am reading the
articles, hope will come over the problem.

Thank you for the follow-up. The article may help, but I think an easy work-around is to try the red channel instead of green, if that is an option. Autofluorescence is generally less of an issue in the red, but you will need a red secondary, such as a...

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Interested in using ab63983 on frozen mouse brain tissue sections.

Thank you for your call today and for your questions. Please see below for information about our testing discount program, and let me know if you have any further questions about these antibodies or our IHC protocols.

Sinceab63983 has not be...

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Can Goat polyclonal Secondary Antibody to Mouse IgA - alpha chain (FITC) (ab97234) be used as seondary antibody for "Anti-Francisella tularensis LPS antibody [FB11] (ab2033) in indirect immunofluorescence assay?

Thank you for your inquiry.


We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


I would reccomend using...

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I want to do an IF application on mouse tissue using a mouse anti-Helicobacter pylori antibody as primary antibody and a goat polyclonal secondary antibody to mouse IgG (FITC conjugated). Do you think it should work? I would like to thank you in advanc...

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Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse ...

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I am writing regarding the product with catalogue number ab6785, We are planning to run a western blotting experiment using ABcam primary antibodies and also using secondary antibody with the cat number ab6785. However we thought this ...

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Thank you for contacting us. The x-ray film may be able to detect fluorescence emission, but the problem is that the FITC would need to be excited with light to emit light, and that would presumably expose all of the film to the excitation light. Lumin...

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Dear Sir/Madam I am writing regarding the product with catalogue number ab6785, We are planning to run a western blotting experiment using ABcam primary antibodies and also using secondary antibody with the cat number ab6785. However we thought this se...

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Thank you for contacting Abcam. The product ab6785, the goat polyclonal secondary antibody to mouse IgG - H&L is conjugated with FITC. The only way to detect FITC is with a laser that has the ability to Excite at 494nm and a filter that can detect Emis...

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