Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
Overview
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Product name
Goat Anti-Mouse IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native Mouse IgG (purified).
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 15767251
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Flow Cyt | (1) |
1/1000 - 1/2000.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. PubMed: 15767251 |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
1/1000 - 1/2000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells
LAMP1 was detected with a mouse antiLAMP1 antibody in ICC/IF analysis of BS-C-1 cells.
Anti-LAMP1 was visualized using ab7064 at 1/500 diulution.
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Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab7064 has been referenced in 26 publications.
- Xu Z et al. Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca2+ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia. Exp Ther Med 21:322 (2021). PubMed: 33732295
- Gallo E et al. Peptide-Based Hydrogels and Nanogels for Delivery of Doxorubicin. Int J Nanomedicine 16:1617-1630 (2021). PubMed: 33688182
- Kanat ÖN & Selmanoglu G Neurotoxic Effect of Fipronil in Neuroblastoma SH-SY5Y Cell Line. Neurotox Res 37:30-40 (2020). PubMed: 31482323
- Smaldone G et al. KCTD15 Protein Expression in Peripheral Blood and Acute Myeloid Leukemia. Diagnostics (Basel) 10:N/A (2020). PubMed: 32512747
- Batir MB et al. Evaluation of the CRISPR/Cas9 directed mutant TP53 gene repairing effect in human prostate cancer cell line PC-3. Mol Biol Rep 46:6471-6484 (2019). PubMed: 31571107