Overview

  • Product name

    Goat Anti-Mouse IgG H&L (Glucose Oxidase)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    ab136783 does not cross react with Human IgG, A and M.
  • Tested applications

    Suitable for: ELISA, IHC-Fr, IHC-P, WB, ICCmore details
  • Minimal
    cross-reactivity


    Human more details
  • Immunogen

    Highly purified whole Mouse immunoglobulin.

  • Conjugation

    Glucose Oxidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136783 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/1000. Substrate:10 mg/ml D(+)glucose, 0.1 mg/ml grade II (Rz=0.6) peroxidase, 0.35 mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
IHC-Fr 1/10 - 1/100.
IHC-P 1/10 - 1/100.
WB 1/400.
ICC 1/10 - 1/100. Substrate: 15 mg/ml D(+)glucose, 0.5 mg/ml blue thiazolyl, 0.35 mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

References

This product has been referenced in:

  • Yu W  et al. Highly sensitive visual detection of amantadine residues in poultry at the ppb level: A colorimetric immunoassay based on a Fenton reaction and gold nanoparticles aggregation. Anal Chim Acta 1027:130-136 (2018). Mouse . Read more (PubMed: 29866262) »
See 1 Publication for this product

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