Nanog Western Blot

Excellent
Abreviews
Application
Western blot
Whole Cell lysate - human ES cells.
Loading amount: 10 µg.
Gel Running Conditions: Reduced Denaturing (10%).
Blocking step: 5%BSA/1%OvaAlb/PBS as blocking agent for 3 hours at room temperature.
Primary antibody: Nanog diluted 1:1000 in 1%BSA/0.2%OvaAlb/PBS 13hours at 4°C.
Secondary antibody: ab205719 - Goat Anti-Mouse IgG H&L (HRP) diluted 1:5000 in TBST.
ECL prime: 4 second exposure.
Predicted size: ~45kDa.

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Submitted Aug 12 2016

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