Overview

  • Product name
    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500 - 1/2500.
IP Use at an assay dependent concentration. PubMed: 17164758
Dot blot Use at an assay dependent concentration.
ELISA 1/100000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/10000. PubMed: 17413034

Images

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

    See Abreview

  • Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

    ab49969 Immunoprecipitating HA Tag in human 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
    Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
    Ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

References

This product has been referenced in:
  • Zhang F  et al. Ligand Activation of PPAR? by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1a. Theranostics 8:610-626 (2018). Read more (PubMed: 29344293) »
  • Penfornis P  et al. Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA. Sci Rep 8:1720 (2018). Read more (PubMed: 29379063) »

See all 194 Publications for this product

Publishing research using ab6789? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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30 reviews or Q&A

10
Votes: Highest First

EXCELLENT ANTI-MOUSE SECONDARY ANTIBODY

 Excellent
Abreviews
Application
Western blot
Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody.

gel: 10%, 200 V for 35 min.

transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.
abreview image
Username

Vicky

Verified customer

Submitted Nov 07 2016

Thank you for your mail.

The customer wants the replacement for these below antibodies.

ab6789, ab72439 & ab77450.

kindly arrange to include these requested FOCRs in the next direct shipment of NuLife.

Lo...

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Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ...

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This is in continuation to the below complaint.

The customer has failed to get positive results from these antibodies, even after following the suggestions made by you. So, the customer is asking for the replacement asap.

Kindly a...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.

I appreciate the time the customer has spent on these experiments, and ...

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It would be appreciated if you can send it to our address in France then we can arrange a delivery safely to the customer.

Sincerely Yours

Thank you for your response.

I have spoken to my colleague and we can certainly ship the replacement vial directly to your company Qafqazbiotex Co to Georgia. Once you place a new order, we can add this item (free of charge)(ab6789) to your d...

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Hi,
Here you are:

Please do not hesitate to contact us if you require any further information.

Best Regards


ORDER #1109926

Thank you for your response and for confirming the order number.

I could arrange a new vial of ab6789 as a free of charge replacement or a credit note. Please could you discuss it with your troubled customer and let me know how you wish to pr...

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Hello,

Following your email, here it is related lot number. Hope it would helpful for you.

Lot. Number GR23382-6

Sincerely Yours

Thank you for confirming the batch number.

In my previous e-mail, I have also asked you to provide your Abcam Order number (or your Purchase Order Number) with the date of purchase. We do not have any order in our system from Georgia so it wo...

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To whom it may concern,,

We found in some cases (such asAB6789)the antibodies inside the tubes are in a gel form. What's the explanation for this if customer asks us in that regards. Is these kind of gel forms are normal?

I am eag...

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Thank you for your enquiry regarding ab6789.

In order to take this complaint further, could you please confirm the batch number and your Abcam Order number (or your Purchase Order Number) with the date of purchase.

As our Abpromise ...

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Needs a streptavidin-FITC protein.

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that thi...

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Needs a streptavidin-FITC protein.

Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happ...

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Dear Technical Team,

Thank you for your mail.

Please find below the reply from the customer on your further queries from him.

Looking forward towards your valued reply on it.

With Best Regards,

Ant...

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Thank you for your message and I appreciate the cooperation from the customer. It is regrettable that three antibodies have not worked for them.

I look forward to hearing from you with the results of the suggestions made. I hope these will h...

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1-10 of 30 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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