Goat Anti-Mouse IgG H&L (HRP) (ab6789)

Reviews (1)Q&A (29)References (260)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500 - 1/2500.
IP Use at an assay dependent concentration. PubMed: 17164758
Dot blot Use at an assay dependent concentration.
ELISA 1/100000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/10000. PubMed: 17413034

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

    All lanes : Cytosolic fraction prepared from murine brain tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 194 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

    ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
    Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)

    ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

References

This product has been referenced in:
  • Zhang F  et al. Ligand Activation of PPAR? by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1a. Theranostics 8:610-626 (2018). Read more (PubMed: 29344293) »
  • Penfornis P  et al. Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA. Sci Rep 8:1720 (2018). Read more (PubMed: 29379063) »
See all 260 Publications for this product

Publishing research using ab6789? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 30 Abreviews or Q&A

EXCELLENT ANTI-MOUSE SECONDARY ANTIBODY

 Excellent
Abreviews
abreview image
Application
Western blot
Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody.

gel: 10%, 200 V for 35 min.

transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.

Vicky

Verified customer

Submitted Nov 07 2016

Question

Thank you for your mail.

The customer wants the replacement for these below antibodies.

ab6789, ab72439 & ab77450.

kindly arrange to include these requested FOCRs in the next direct shipment of NuLife.

Looking forward towards your valued confirmation on it.

With Best Regards,

Read More
Answer

Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ab77450 have been added to your order number [BO] NL-396-Dt:-11-12-2012. This has Abcam order reference 1224924.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vials, or if you have any further questions, please do not hesitate to let me know.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

Read More

Question

This is in continuation to the below complaint.

The customer has failed to get positive results from these antibodies, even after following the suggestions made by you. So, the customer is asking for the replacement asap.

Kindly advise, enabling us to update the customer on it.

With Best Regards,

Read More
Answer

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.

I appreciate the time the customer has spent on these experiments, and would like to offer a refund or credit note in compensation (providing the items have been purchased in the 6 month guarantee period). I am sorry I notice I do no think we have received the order numbers in this case? To enable us to proceed with this, I would appreciate if you could please confirm the order numbers and date of purchase?

Please note that it is important for us to receive the order number as soon as possible when a complaint is submitted, preferably within the first email. This is vital for our investigation of the case and for our quality monitoring purposes.

Thank you for your continued cooperation. I look forward to hearing from you with the order information and details of how you would like to proceed.

Read More

Question

It would be appreciated if you can send it to our address in France then we can arrange a delivery safely to the customer.

Sincerely Yours

Read More
Answer

Thank you for your response.

I have spoken to my colleague and we can certainly ship the replacement vial directly to your company Qafqazbiotex Co to Georgia. Once you place a new order, we can add this item (free of charge)(ab6789) to your document and despatch them together. However, we are unable to send the product via France.

Let me know if you have any further question and when the new order will be ready.

Read More

Question

Hi,
Here you are:

Please do not hesitate to contact us if you require any further information.

Best Regards


ORDER #1109926

Read More
Answer

Thank you for your response and for confirming the order number.

I could arrange a new vial of ab6789 as a free of charge replacement or a credit note. Please could you discuss it with your troubled customer and let me know how you wish to proceed with this enquiry.

I look forward to hearing from you soon.

Read More

Question

Hello,

Following your email, here it is related lot number. Hope it would helpful for you.

Lot. Number GR23382-6

Sincerely Yours

Read More
Answer

Thank you for confirming the batch number.

In my previous e-mail, I have also asked you to provide your Abcam Order number (or your Purchase Order Number) with the date of purchase. We do not have any order in our system from Georgia so it would be interesting to know how you purchased this product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

I look forward to hearing from you soon.

Read More

Question

To whom it may concern,,

We found in some cases (such asAB6789)the antibodies inside the tubes are in a gel form. What's the explanation for this if customer asks us in that regards. Is these kind of gel forms are normal?

I am eagerly looking forward to hearing from you,

Sincerely Yours

Read More
Answer

Thank you for your enquiry regarding ab6789.

In order to take this complaint further, could you please confirm the batch number and your Abcam Order number (or your Purchase Order Number) with the date of purchase.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

I look forward to hearing from you soon.

Read More

Question

Needs a streptavidin-FITC protein.

Read More
Answer

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that this took longer than expected, but I hope that this information will be useful. Please let me know if you have any questions and I'll be happy to help.

Read More

Question

Needs a streptavidin-FITC protein.

Read More
Answer

Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happen within the next couple of days.

Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Question

Dear Technical Team,

Thank you for your mail.

Please find below the reply from the customer on your further queries from him.

Looking forward towards your valued reply on it.

With Best Regards,

Antibody 1 ab72439



1. How has this antibody been stored? -20 degree C after received from vendor

2. Which species is the brain tissue from? rat brain

3. Is the secondary antibody working well with other primary antibodies? What
is the supplier and product number? Yes, supplier Millipore, No:AP307P

4. I can recommend to incubate overnight at4oC. This can often
provide more specific and efficient binding.

5. Has the transfer to the membrane and quality of the sample been assessed
using a loading control? yes



Antibody 2 ab6789

1.
Which species was the sample from? rat brain

2. How has this antibody been stored? -20 degree C after received from vendor

3. Is the primary GAPDH antibody working well with other secondary antibodies? yes, it worked
perfectly with secondary gt anti ms from santacruz biotech.

4. I can recommend to incubate overnight at4oC. This can often
provide more specific and efficient binding.

5. Has the transfer to the membrane and quality of the sample been assessed
using a loading control? yes



Antibody 3 ab77450

1. I can confirm this antibody is not tested in perfusion fixed sections, but
because it has been tested in IHC-P and IHC-Fr which are very similar, I can
provide an exception for the guarantee in this case.

2. I can recommend to try different times for antigen retrieval, such as 3. 5
and10 minutes. This can sometimes require some optimization, and shorter
time can sometimes be better.

3.Triton is a strong detergent and can sometimes disrupt protein
epitopes. I can suggest to try a more gentle detergent such as 0.2% Tween 20.

I will contact again after trying the recommendations. But I think first two antibody need to be replaced. For third one I am changing triton to tween 20 and antigen retrival time. Lets see what happen.

Thanks

Read More
Answer

Thank you for your message and I appreciate the cooperation from the customer. It is regrettable that three antibodies have not worked for them.

I look forward to hearing from you with the results of the suggestions made. I hope these will help improve the results. Please do not hesitate to contact me with further details if there are still any concerns.

Read More

1-10 of 30 Abreviews or Q&A

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