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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (HRP) (ab6789)

  • Datasheet
  • SDS
Reviews (1)Q&A (29)References (576)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
  • Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    mouse IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

Associated products

  • Related Products

    • Normal Goat Serum (ab7481)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/500 - 1/2500.
IP
Use at an assay dependent concentration. PubMed: 17164758
Dot blot
Use at an assay dependent concentration.
ELISA
1/100000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
Immunomicroscopy
Use at an assay dependent concentration.
WB (1)
1/2000 - 1/10000. PubMed: 17413034
Notes
ICC
1/500 - 1/2500.
IP
Use at an assay dependent concentration. PubMed: 17164758
Dot blot
Use at an assay dependent concentration.
ELISA
1/100000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/2000 - 1/10000. PubMed: 17413034

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

    All lanes : Cytosolic fraction prepared from murine brain tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 194 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

    ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

    ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (576)

Publishing research using ab6789? Please let us know so that we can cite the reference in this datasheet.

ab6789 has been referenced in 576 publications.

  • Li L  et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). PubMed: 31270802
  • Guo Z  et al. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med 45:141-150 (2020). PubMed: 31746344
  • Martínez Corrales G  et al. Partial Inhibition of RNA Polymerase I Promotes Animal Health and Longevity. Cell Rep 30:1661-1669.e4 (2020). PubMed: 32049000
  • Zhang HH  et al. miR-21 inhibits autophagy and promotes malignant development in the bladder cancer T24 cell line. Int J Oncol 56:986-998 (2020). PubMed: 32319564
  • Huang H  et al. The MicroRNA MiR-29c Alleviates Renal Fibrosis via TPM1-Mediated Suppression of the Wnt/ß-Catenin Pathway. Front Physiol 11:331 (2020). PubMed: 32346368
View all Publications for this product

Customer reviews and Q&As

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EXCELLENT ANTI-MOUSE SECONDARY ANTIBODY

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody.

gel: 10%, 200 V for 35 min.

transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.

Vicky

Verified customer

Submitted Nov 07 2016

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