Goat Anti-Mouse IgG H&L (HRP) (ab6789)
- Datasheet
- References (443)
- Protocols
Overview
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Product name
Goat Anti-Mouse IgG H&L (HRP)
See all IgG secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
Immunogen
mouse IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
General notes
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support. -
Research areas
Associated products
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Related Products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab6789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/500 - 1/2500. | |
| IP | Use at an assay dependent concentration. PubMed: 17164758 | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/100000. | |
| IHC-P | 1/500 - 1/2500. | |
| IHC-Fr | 1/500 - 1/2500. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/10000. PubMed: 17413034 |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution
All lanes : Cytosolic fraction prepared from murine brain tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 194 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
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Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
References
This product has been referenced in:
- Li L et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). Read more (PubMed: 31270802) »
- Zhang L et al. Progesterone receptor antagonist provides palliative effects for uterine leiomyoma through a Bcl-2/Beclin1-dependent mechanism. Biosci Rep 39:N/A (2019). Read more (PubMed: 31262976) »
