Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.05% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000.
WB 1/2000 - 1/20000.

Images

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97023, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References

This product has been referenced in:

  • Seto Y  et al. Strigolactone perception and deactivation by a hydrolase receptor DWARF14. Nat Commun 10:191 (2019). Read more (PubMed: 30643123) »
  • Huang Y  et al. Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5. Front Neurol 10:32 (2019). Read more (PubMed: 30778327) »
See all 108 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Secondary antibody (ab97023) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for Ikb alpha antibody from non abcam brand. Thought the secondary antibody could detect a band at expected 42 kD,
There was a non specific band (at 55 kD was shown by the secondary antibody when used without primary antibody incubation also.
gel: 12%, 200 V for 45 min.
transfer 17 V for 35 min. using Biorad SD.
Blocking 5% BSA.

Abcam user community

Verified customer

Submitted Oct 10 2016

Abreviews
Application
Western blot
1. The concentration of Goat Anti-Mouse IgG H&L (HRP) used for this experiment is 1:10000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.

Mr. Lenin Veeraval

Verified customer

Submitted Jun 18 2015

Abreviews
Application
Western blot
Used at 1:5000 and works excellently every time.

Abcam user community

Verified customer

Submitted Aug 15 2014

Question
Answer

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

ab97023 - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

ab97040 - Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

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Answer

Thank you for contacting us.
The ab97023 and ab97046 are the antibodies from our AbExcel range; they are extensively tested, can be routinely used at dilutions ranging from 1/2000 to 1/20,000 and based on a 1/2000 dilution in 3ml milk/BSA, you can do over 600 blots with a single vial of HRP-conjugated AbExcel secondary. These are the reasons, why I recommended these products.
We have not performed western blot with IP lysates so we are unsure about the specificity towards native or denatured chains.
I can reassure you that these products are top of the range products and comes with full Abpromise guarantee. If because of any reason you are not satisfied with results please let us know we will help you with troubleshooting. Refund or credit note can also be given if you ended up being not happy at all with results.

I hope this information is helpful to you. We will look forward to receiving your order soon.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.
We have better antibodies than ebioscience and these are stringently tested to get best results and best value per blot. Please click the following link for datasheet;
https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-mouse-igg-h-l-hrp-ab97046.html
https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-HRP-ab97023.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to understand what may be contributing to this problem more fully, could you please fill out the questionnaire I have attached to this email. This should only take 5-10 minutes to complete. OnceI have receive the questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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