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Goat Anti-Mouse IgG H&L (HRP) (ab97023)

Reviews (4)Q&A (4)References (235)
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    Part of the AbExcel range.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/5000.
ELISA (1)
1/10000 - 1/100000.
WB (3)
1/2000 - 1/20000.
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000.
WB
1/2000 - 1/20000.

Images

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)This image is courtesy of an anonymous Abreview.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97023, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
    All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (235)

Publishing research using ab97023? Please let us know so that we can cite the reference in this datasheet.

ab97023 has been referenced in 235 publications.

  • Huang KY  et al. Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Mol Med 13:e12828 (2021). PubMed: 33159417
  • Shao J  et al. Exosomes from Kartogenin-Pretreated Infrapatellar Fat Pad Mesenchymal Stem Cells Enhance Chondrocyte Anabolism and Articular Cartilage Regeneration. Stem Cells Int 2021:6624874 (2021). PubMed: 33763141
  • Saxena S  et al. Plexin-B3 Regulates Cellular Motility, Invasiveness, and Metastasis in Pancreatic Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 33669221
  • Yu Q  et al. Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells. J Cell Mol Med 25:1601-1612 (2021). PubMed: 33410267
  • Saulnier RJ  et al. Chronic social stress alters protein metabolism in juvenile rainbow trout, Oncorhynchus mykiss. J Comp Physiol B 191:517-530 (2021). PubMed: 33712903
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Non specific band by secondary antibody without primary antibody incubation.

 Average
Abreviews
abreview image
Application
Western blot
Secondary antibody (ab97023) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for Ikb alpha antibody from non abcam brand. Thought the secondary antibody could detect a band at expected 42 kD,
There was a non specific band (at 55 kD was shown by the secondary antibody when used without primary antibody incubation also.
gel: 12%, 200 V for 45 min.
transfer 17 V for 35 min. using Biorad SD.
Blocking 5% BSA.

Abcam user community

Verified customer

Submitted Oct 10 2016

Western Blot on Neuro2A cells

 Good
Abreviews
abreview image
Application
Western blot
1. The concentration of Goat Anti-Mouse IgG H&L (HRP) used for this experiment is 1:10000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.

MR. Lenin Veeraval

Verified customer

Submitted Jun 18 2015

Detection of USP14 (ab56427) in mES cells

 Excellent
Abreviews
abreview image
Application
Western blot
Used at 1:5000 and works excellently every time.

Abcam user community

Verified customer

Submitted Aug 15 2014

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