Application
Western blot
Sample
Mouse Tissue lysate - whole (adipocyte tissue)
Loading amount
35 µg
Specification
adipocyte tissue
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-tween
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~50 kDa Non-specific: 35 kDa
Positive control
adiptocyte tissue from ob/ob mice on high fat diet
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Oct 18 2012