Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP polymer)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Based on IEP, ab214879 reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit.
  • Tested applications

    Suitable for: Immunoelectrophoresis, IHC-Pmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG. (Purified Mouse IgG, whole molecule).

  • Conjugation

    HRP polymer

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 1% BSA, 98% PBS

    ab214879 is liquid with light red color.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab214879 was affinity purified using solid phase Mouse IgG. Purity >95% by SDS-PAGE.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunoelectrophoresis Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.

Images

  • Immunohistochemical analysis of Triple positive breast cancer tissue stained with Ki-67 and detected with ab214879.

References

This product has been referenced in:

  • Zhang C  et al. FOXA1 is upregulated in glioma and promotes proliferation as well as cell cycle through regulation of cyclin D1 expression. Cancer Manag Res 10:3283-3293 (2018). Read more (PubMed: 30233244) »
  • Fang L  et al. Resistance to epithelial growth factor receptor tyrosine kinase inhibitors in a patient with transformation from lung adenocarcinoma to small cell lung cancer: A case report. Oncol Lett 14:593-598 (2017). Read more (PubMed: 28693210) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE human tissue sections, the FFPE human skin tumor section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.
The staining works well.

Abcam user community

Verified customer

Submitted Jul 30 2018

Application
Immunohistochemistry paraffin embedded sections
In this study to test how well the goat anti-mouse IgG (HRP polymer) works on FFPE pig tissue sections, the FFPE human pig lung section was deparaffinized and boiled in antigen retrieval buffer, 10mM Sodium Citrate pH6. Overnight incubation in a mouse anti-Smooth muscle actin primary antibody from Sigma Aldrich. The goat anti-mouse IgG (HRP polymer) secondary antibody is used at 1:1 dilution in 2% BSA for 90 minutes at room temperature. The slides were then developed in DAB solution and counter-stained with hematoxylin.

The staining works well. The picture enclosed shows clear SMA staining in pig lung and a negative control insert is included for reference.

Abcam user community

Verified customer

Submitted Jul 30 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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