Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab7068)

Submit a review Q&A (1)References (10)

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: Immunomicroscopy, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WB

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: Immunomicroscopy, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
WB
Notes
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/12000 - 1/60000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (10)

    Publishing research using ab7068? Please let us know so that we can cite the reference in this datasheet.

    ab7068 has been referenced in 10 publications.

    • Denu RA  et al. Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Mol Cancer Res 18:33-45 (2020). PubMed: 31604847
    • Yu L & Qian J Dihydrotanshinone I Alleviates Spinal Cord Injury via Suppressing Inflammatory Response, Oxidative Stress and Apoptosis in Rats. Med Sci Monit 26:e920738 (2020). PubMed: 32112706
    • Kramarski L & Arbely E Translational read-through promotes aggregation and shapes stop codon identity. Nucleic Acids Res 48:3747-3760 (2020). PubMed: 32128584
    • Cohen S  et al. Nonsense mutation-dependent reinitiation of translation in mammalian cells. Nucleic Acids Res 47:6330-6338 (2019). PubMed: 31045216
    • Belo Y  et al. Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine. Biochim Biophys Acta Gen Subj 1863:1343-1350 (2019). PubMed: 31170499
    • Ferdous S  et al. Characterization of LSD1 Expression Within the Murine Eye. Invest Ophthalmol Vis Sci 60:4619-4631 (2019). PubMed: 31675426
    • Gibori H  et al. Amphiphilic nanocarrier-induced modulation of PLK1 and miR-34a leads to improved therapeutic response in pancreatic cancer. Nat Commun 9:16 (2018). WB ; Human . PubMed: 29295989
    • Lv T  et al. Zerumbone suppresses the potential of growth and metastasis in hepatoma HepG2 cells via the MAPK signaling pathway. Oncol Lett 15:7603-7610 (2018). PubMed: 29849796
    • Muzika M  et al. Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins. RSC Adv 8:25558-25567 (2018). PubMed: 30713681
    • Kfir N  et al. SF3B1 association with chromatin determines splicing outcomes. Cell Rep 11:618-29 (2015). PubMed: 25892229

    Customer reviews and Q&As

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    Question

    I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


    Thanks,

    Read More
    Answer

    Thank you for contacting us.

    The following products would be suitable as per your requirements. The catalogue numbers are

    ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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