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  1. Link

    goat-mouse-igg-hl-hrp-preadsorbed-ab97040.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

  • Datasheet
  • SDS
Reviews (2)Q&A (11)References (169)

Product price, shipping and contact information

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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, PBS, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

Associated products

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000.
WB (2)
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.
Notes
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000.
WB
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (169)

Publishing research using ab97040? Please let us know so that we can cite the reference in this datasheet.

ab97040 has been referenced in 169 publications.

  • Zhang Y  et al. Duck enteritis virus infection suppresses viability and induces apoptosis and endoplasmic reticulum stress in duck embryo fibroblast cells via the regulation of Ca2. J Vet Med Sci 83:549-557 (2021). PubMed: 33116004
  • Zeng W  et al. Lidocaine suppresses the malignant behavior of gastric cancer cells via the c-Met/c-Src pathway. Exp Ther Med 21:424 (2021). PubMed: 33747163
  • Huang Y  et al. Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis. Hum Cell 34:1244-1254 (2021). PubMed: 33999360
  • Liu K  et al. Single-cell analysis reveals metastatic cell heterogeneity in clear cell renal cell carcinoma. J Cell Mol Med 25:4260-4274 (2021). PubMed: 33759378
  • Jiang L  et al. Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncol Lett 21:109 (2021). PubMed: 33376542
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 13 Abreviews or Q&A

secondary anti-mouse in WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
the antibody gives clear bands with the primaries. I usually use it in 1:2000-3000 dilution.
Image: 2ry anti mouse after 1ry mouse GAPDH, replicates of macrophages whole cell lysate

Abcam user community

Verified customer

Submitted Jul 03 2019

Good abtibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
I use this antibody mostly in WB. I can get good signals(1/10000 dilution).

Upload image condition:
1st antibody ab8245 1/5000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)

Abcam user community

Verified customer

Submitted Oct 02 2017

Question

I am just wondering what is the difference between ab97023 and ab97040?

Read More

Abcam community

Verified customer

Asked on Jan 28 2013

Answer

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

ab97023 - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

ab97040 - Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Read More

Abcam Scientific Support

Answered on Jan 28 2013

Question

Inquiry: Hello I have buyed a primary antibody of BANF-1 and secondary antibody (ab97040)from your company.Now i am ready to starting IHC to detect the antigen's expression of human's thymus.I have many questions to ask you:1.The best method of antigen repairing.Enzyme or mircowave oven etc.2.The incubation time and temperature of primary antibody.3.The best diultion of secondary antibody.4.The buffer to diluent the primary and secondary antibody. Thank you very much for helping me.

Read More

Abcam community

Verified customer

Asked on Nov 15 2012

Answer

Thank you for contacting us.

Fortunately we offer various protocols and tips that can be found on our webpage under scientific support protocols.

I will give you some links that might be useful for your experiments. However, please note that every antibody and every experiment needs some optimisation. The protocols are just general guidelines.


1. https://docs.abcam.com/pdf/protocols/Permeabilization-and-fixation.pdf

2. https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf (for your questions 1-5)

3. https://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf

4. https://www.abcam.com/ps/pdf/protocols/abcam_troubleshooting_tips_IHC.pdf


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 15 2012

Question

Dear ,

The order reference is: 1xxxx
and it was received on the 2/2/12

Thank you

Read More

Abcam community

Verified customer

Asked on Apr 05 2012

Answer

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.

Hopefully, this new secondary antibody will help the situation. However, if you find the same all over staining please do let me know and we'll take it one reagent at a time to isolate the step which is causing the problems.

I look forward to hearing how you get on.

Read More

Abcam Scientific Support

Answered on Apr 05 2012

Question

Thank you very much for your help. Aside from your secondary antibody i don't have another to hand i can use. I will look into another detection method, but as i have just started research i'm just a little unsure and unconfident about it. Thank you very much for your support, i really appreciate it, for you to provide another secondary antibody it would help me greatly.

Read More

Abcam community

Verified customer

Asked on Mar 30 2012

Answer

Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.

Once I have this information I will look into an appropriate alternative to try.

I look forward to receiving your reply.

Read More

Abcam Scientific Support

Answered on Mar 30 2012

Question

We need the COA and MSDS for the following products , so kindly forward the COA and MSDS at the earliest possible.

Read More

Abcam community

Verified customer

Asked on Feb 14 2012

Answer

Thank you for your enquiry.

Please find attached the MSDS for ab97040, which can also be downloaded from the datasheet of this antibody.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within six months of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would like to receive a CoC? If so, could you please confirm the lot number? Unfortunately, x is not a valid lot number for ab97040.

Please do not hesitate to contact me again if you have further questions.

Read More

Abcam Scientific Support

Answered on Feb 14 2012

Question

Thank you for your reply. I am sorry to disturb you again. We have not received the shipment of ab7061 yet. Due to the experimental requirement, he needs ab7061 to continue. I am very sorry, and please accept our apology for any inconvenience this situation may have caused you. Would you please help us deal with this issue?  

Read More

Abcam community

Verified customer

Asked on Dec 14 2011

Answer

Again I apologize for this. As you know these replacement vials are typically sent off very quickly. I have requested (again) that this replacement vial be shipped immediately. I have just received a reply from our UK office stating they are now aware that no replacement has been sent yet. It seems my two inital requests somehow slipped through the cracks. I am optimistic that this time the vial of ab7061 will be sent in the next shipment to Interlab. I am very sorry for this extended delay to you and your customer. I will update you with any further details provided to me. Thanks so much for your patience!

Read More

Abcam Scientific Support

Answered on Dec 14 2011

Question

Thank you for your help. Sorry to disturb you. This customer would like to inquire when he could get the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him deal with this issue?  

Read More

Abcam community

Verified customer

Asked on Dec 07 2011

Answer

Thanks for your emai. I am sorry you have not received the shipment yet.  I requested a replacement of ab7061 be sent on Dec.1. I am looking into this issue and will let you know when to expect this antibody. Thanks for your patience.

Read More

Abcam Scientific Support

Answered on Dec 07 2011

Question

Thank you for your kind help. After contacting this customer, he would like to receive the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him provide the replacement?  

Read More

Abcam community

Verified customer

Asked on Dec 01 2011

Answer

I have requested that a vial of ab7061 be included in the next shipment to your company for this customer. I hope this is helpful. Please contact me again if you have any further questions.

Read More

Abcam Scientific Support

Answered on Dec 01 2011

1-10 of 13 Abreviews or Q&A

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