Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
Key features and details
- Goat Anti-Mouse IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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Tested applications
Suitable for: IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details -
Immunogen
Other Immunogen Type corresponding to Mouse IgG.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, PBS, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/5000.
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| ELISA |
1/10000 - 1/100000.
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| WB | (2) |
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.
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| Notes |
|---|
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IHC-P
1/200 - 1/5000. |
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ELISA
1/10000 - 1/100000. |
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WB
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (166)
ab97040 has been referenced in 166 publications.
- Zhang Y et al. Duck enteritis virus infection suppresses viability and induces apoptosis and endoplasmic reticulum stress in duck embryo fibroblast cells via the regulation of Ca2. J Vet Med Sci 83:549-557 (2021). PubMed: 33116004
- Zeng W et al. Lidocaine suppresses the malignant behavior of gastric cancer cells via the c-Met/c-Src pathway. Exp Ther Med 21:424 (2021). PubMed: 33747163
- Huang Y et al. Long non-coding RNA TPT1-AS1 sensitizes breast cancer cell to paclitaxel and inhibits cell proliferation by miR-3156-5p/caspase 2 axis. Hum Cell 34:1244-1254 (2021). PubMed: 33999360
- Liu K et al. Single-cell analysis reveals metastatic cell heterogeneity in clear cell renal cell carcinoma. J Cell Mol Med 25:4260-4274 (2021). PubMed: 33759378
- Jiang L et al. Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncol Lett 21:109 (2021). PubMed: 33376542