Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

Abreviews (1)Q&A (11)Specific References (28)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.

Images

  • Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040)
    Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040)

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) was used at 1/5000 dilution developed using the ECL technique, with ab86741.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97040, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Yang GS  et al. mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia. Mol Med Rep 17:5821-5829 (2018). Read more (PubMed: 29484389) »
  • Pan H  et al. Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy. Int J Nanomedicine 13:3189-3201 (2018). Read more (PubMed: 29881272) »

See all 28 Publications for this product

Publishing research using ab97040? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First

Good abtibody

 Excellent
Abreviews
Application
Western blot
I use this antibody mostly in WB. I can get good signals(1/10000 dilution).

Upload image condition:
1st antibody ab8245 1/5000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)
abreview image
Username

Abcam user community

Verified customer

Submitted Oct 02 2017

I am just wondering what is the difference between ab97023 and ab97040?

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

ab97023 - This antibody was isolated by affini...

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Inquiry: Hello I have buyed a primary antibody of BANF-1 and secondary antibody (ab97040)from your company.Now i am ready to starting IHC to detect the antigen's expression of human's thymus.I have many questions to ask you:1.The best method of antigen...

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Thank you for contacting us.

Fortunately we offer various protocols and tips that can be found on our webpage under scientific support protocols.

I will give you some links that might be useful for your experiments. However, please n...

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Dear ,

The order reference is: 1xxxx
and it was received on the 2/2/12

Thank you

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.
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Thank you very much for your help. Aside from your secondary antibody i don't have another to hand i can use. I will look into another detection method, but as i have just started research i'm just a little unsure and unconfident about it. Thank you ve...

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Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.
...

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We need the COA and MSDS for the following products , so kindly forward the COA and MSDS at the earliest possible.

Thank you for your enquiry.

Please find attached the MSDS for ab97040, which can also be downloaded from the datasheet of this antibody.

Some of our antibodies are produced in-house and others are sourced from suppliers or academi...

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Thank you for your reply. I am sorry to disturb you again. We have not received the shipment of ab7061 yet. Due to the experimental requirement, he needs ab7061 to continue. I am very sorry, and please accept our apology for any inconvenience this situ...

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Again I apologize for this. As you know these replacement vials are typically sent off very quickly. I have requested (again) that this replacement vial be shipped immediately. I have just received a reply from our UK office stating they are now aware ...

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Thank you for your help. Sorry to disturb you. This customer would like to inquire when he could get the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him deal with this issue?

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Thanks for your emai. I am sorry you have not received the shipment yet.  I requested a replacement of ab7061 be sent on Dec.1. I am looking into this issue and will let you know when to expect this antibody. Thanks for your patience.

Thank you for your kind help. After contacting this customer, he would like to receive the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him provide the replacement?

I have requested that a vial of ab7061 be included in the next shipment to your company for this customer. I hope this is helpful. Please contact me again if you have any further questions.

Phone call: Customer kindly requested the MSDS.

Thank you for contacting us. Please find attached. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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