Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

Reviews (2)Q&A (11)References (87)
Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details

  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, PBS, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000. (Primary).
WB (2)
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.
Notes
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.

Images

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 150 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using an anti-mouse HRP (ab97040), and visualised using ECL development solution ab133406

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) was used at 1/5000 dilution developed using the ECL technique, with ab86741.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97040, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (87)

Publishing research using ab97040? Please let us know so that we can cite the reference in this datasheet.

ab97040 has been referenced in 87 publications.

  • Li Y  et al. LncRNA-LET relieves hypoxia-induced injury in H9c2 cells through regulation of miR-138. J Cell Biochem 121:259-268 (2020). PubMed: 31222827
  • Dong L  et al. Long non-coding RNA GACAT3 promotes liver cancer progression by regulating the proliferation, apoptosis and migration of tumor cells. Exp Ther Med 19:3377-3383 (2020). PubMed: 32266036
  • Chanyshev MD  et al. Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. Toxicol Mech Methods N/A:1-8 (2020). PubMed: 32508177
  • Yan B  et al. Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression. Biomed Res Int 2020:2753414 (2020). PubMed: 32337233
  • Yu S  et al. Long noncoding RNA H19 promotes chemotherapy resistance in choriocarcinoma cells. J Cell Biochem N/A:N/A (2019). PubMed: 31020694
View all Publications for this product

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

secondary anti-mouse in WB

 Excellent
Abreviews
abreview image
Application
Western blot
the antibody gives clear bands with the primaries. I usually use it in 1:2000-3000 dilution.
Image: 2ry anti mouse after 1ry mouse GAPDH, replicates of macrophages whole cell lysate

Abcam user community

Verified customer

Submitted Jul 03 2019

Good abtibody

 Excellent
Abreviews
abreview image
Application
Western blot
I use this antibody mostly in WB. I can get good signals(1/10000 dilution).

Upload image condition:
1st antibody ab8245 1/5000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)

Abcam user community

Verified customer

Submitted Oct 02 2017

Question

I am just wondering what is the difference between ab97023 and ab97040?

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Answer

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

ab97023 - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

ab97040 - Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

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Question

Inquiry: Hello I have buyed a primary antibody of BANF-1 and secondary antibody (ab97040)from your company.Now i am ready to starting IHC to detect the antigen's expression of human's thymus.I have many questions to ask you:1.The best method of antigen repairing.Enzyme or mircowave oven etc.2.The incubation time and temperature of primary antibody.3.The best diultion of secondary antibody.4.The buffer to diluent the primary and secondary antibody. Thank you very much for helping me.

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Answer

Thank you for contacting us.

Fortunately we offer various protocols and tips that can be found on our webpage under scientific support protocols.

I will give you some links that might be useful for your experiments. However, please note that every antibody and every experiment needs some optimisation. The protocols are just general guidelines.


1. https://docs.abcam.com/pdf/protocols/Permeabilization-and-fixation.pdf

2. https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf (for your questions 1-5)

3. https://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf

4. https://www.abcam.com/ps/pdf/protocols/abcam_troubleshooting_tips_IHC.pdf


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Dear ,

The order reference is: 1xxxx
and it was received on the 2/2/12

Thank you

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Answer

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.

Hopefully, this new secondary antibody will help the situation. However, if you find the same all over staining please do let me know and we'll take it one reagent at a time to isolate the step which is causing the problems.

I look forward to hearing how you get on.

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Question

Thank you very much for your help. Aside from your secondary antibody i don't have another to hand i can use. I will look into another detection method, but as i have just started research i'm just a little unsure and unconfident about it. Thank you very much for your support, i really appreciate it, for you to provide another secondary antibody it would help me greatly.

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Answer

Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.

Once I have this information I will look into an appropriate alternative to try.

I look forward to receiving your reply.

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Question

We need the COA and MSDS for the following products , so kindly forward the COA and MSDS at the earliest possible.

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Answer

Thank you for your enquiry.

Please find attached the MSDS for ab97040, which can also be downloaded from the datasheet of this antibody.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within six months of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would like to receive a CoC? If so, could you please confirm the lot number? Unfortunately, x is not a valid lot number for ab97040.

Please do not hesitate to contact me again if you have further questions.

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Question

Thank you for your reply. I am sorry to disturb you again. We have not received the shipment of ab7061 yet. Due to the experimental requirement, he needs ab7061 to continue. I am very sorry, and please accept our apology for any inconvenience this situation may have caused you. Would you please help us deal with this issue?  

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Answer

Again I apologize for this. As you know these replacement vials are typically sent off very quickly. I have requested (again) that this replacement vial be shipped immediately. I have just received a reply from our UK office stating they are now aware that no replacement has been sent yet. It seems my two inital requests somehow slipped through the cracks. I am optimistic that this time the vial of ab7061 will be sent in the next shipment to Interlab. I am very sorry for this extended delay to you and your customer. I will update you with any further details provided to me. Thanks so much for your patience!

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Question

Thank you for your help. Sorry to disturb you. This customer would like to inquire when he could get the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him deal with this issue?  

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Answer

Thanks for your emai. I am sorry you have not received the shipment yet.  I requested a replacement of ab7061 be sent on Dec.1. I am looking into this issue and will let you know when to expect this antibody. Thanks for your patience.

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Question

Thank you for your kind help. After contacting this customer, he would like to receive the replacement with ab7061 (Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed). Would you please help him provide the replacement?  

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Answer

I have requested that a vial of ab7061 be included in the next shipment to your company for this customer. I hope this is helpful. Please contact me again if you have any further questions.

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1-10 of 13 Abreviews or Q&A

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