Question (66835) | Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

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Question

Inquiry: Hello I have buyed a primary antibody of BANF-1 and secondary antibody (ab97040)from your company.Now i am ready to starting IHC to detect the antigen's expression of human's thymus.I have many questions to ask you:1.The best method of antigen repairing.Enzyme or mircowave oven etc.2.The incubation time and temperature of primary antibody.3.The best diultion of secondary antibody.4.The buffer to diluent the primary and secondary antibody. Thank you very much for helping me.

Answer

Thank you for contacting us.

Fortunately we offer various protocols and tips that can be found on our webpage under scientific support protocols.

I will give you some links that might be useful for your experiments. However, please note that every antibody and every experiment needs some optimisation. The protocols are just general guidelines.


1. https://docs.abcam.com/pdf/protocols/Permeabilization-and-fixation.pdf

2. https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf (for your questions 1-5)

3. https://www.abcam.com/ps/pdf/protocols/immunostaining_at_a_glance.pdf

4. https://www.abcam.com/ps/pdf/protocols/abcam_troubleshooting_tips_IHC.pdf


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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