Overview

  • Product name
    Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    This antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA.
  • Tested applications
    Suitable for: In-Cell ELISA, WBmore details
  • Minimal
    cross-reactivity

    Goat, Horse, Human, Rabbit, Rat more details
  • Immunogen

    Full length protein corresponding to Mouse IgG.

  • Conjugation
    IRDye® 800CW

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Affinity chromatography purified using pooled mouse IgG covalently linked to agarose.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In-Cell ELISA 1/200 - 1/1200.
WB 1/5000 - 1/25000.

Images

  • All lanes : Anti-p53 antibody [DO-1] - ChIP Grade (ab1101) at 1/1000 dilution (Anti-p53 antibody [DO-1])

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : p53 knockout HAP1 cell lysate (20 µg)
    Lane 3 : A431 cell lysate (20 µg)
    Lane 4 : Saos-2 cell lysate (20 µg)

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab1101 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - ab1101 observed at 53 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777

  • All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution (Anti-beta Actin antibody [AC-15])

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab6276 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777

  • Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 for detection among its components.
  • Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.

References

This product has been referenced in:
  • Ding M  et al. Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway. Int J Mol Med 42:3571-3581 (2018). Read more (PubMed: 30272282) »
  • Zhong L  et al. Arsenic trioxide induces the apoptosis and decreases NF-?B expression in lymphoma cell lines. Oncol Lett 16:6267-6274 (2018). Read more (PubMed: 30333888) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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